FastQCFastQC Report
Mon 3 Apr 2023
HW22CDRX2_l02.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22CDRX2_l02.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201702015
Sequences flagged as poor quality0
Sequence length10
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCCTTG5155468125.559824476716308No Hit
GGCGACAAGC4421522421.9210621173021No Hit
GGGCGGGGGG151011927.486882072050694No Hit
GGTAGCCTTG148335917.354210616091267No Hit
GGCGCGTCGC82670284.098634314585305No Hit
TTTATCCTTG57013362.826613308746569No Hit
TTCGACAAGC54321842.693172896661444No Hit
GTCGACAAGC47774982.3685921035543447No Hit
GGGCGGGGTG45825592.271945076998859No Hit
GGCGCGACGC43803062.171671909177506No Hit
GTTATCCTTG35352011.7526850190366219No Hit
TTAAACAATC27181221.3475928834920168No Hit
GGTAGGGGTG21680371.0748712649201844No Hit
GGCGGGTCGC15947400.7906415808488576No Hit
GGCGCGAAGC15711290.7789356987831777No Hit
GGGAGCCTTG14812400.7343704523725259No Hit
GGCGAGAAGC14765490.73204474432246No Hit
GGCGCCAAGC14103660.6992324791599132No Hit
GGCGACAATC13625350.675518784480165No Hit
GGGAGGGGTG12964930.6427764244199543No Hit
GGCAACAATC10338240.5125501596996936No Hit
TTCGACAATC7369240.3653528200994918No Hit
GGTCGGGGTG7107840.3523931082195684No Hit
GGGGGGGGGG6908850.3425275647345417No Hit
GGTAACAATC5955350.29525485900574666No Hit
TTCAACAATC4989130.24735152001332264No Hit
TTTATCATTG4930980.24446855426803743No Hit
GGTATCCTGG4880980.24198964992987304No Hit
GTAAACAATC4575430.22684106551935043No Hit
GGTATCATTG4537190.22494519948152228No Hit
GGCGACACGC4433280.21979354048594904No Hit
GGTAGCCGTG4421870.2192278545159799No Hit
GGTCGGGGGG3591150.1780423462799814No Hit
GGTATCCGTG3538200.1754171865858653No Hit
GGCCCGTCGC3258840.16156705226767318No Hit
TTACACAATC3239760.16062110237222965No Hit
TTACACAAGC3154490.1563935789139241No Hit
TTAAACAATG3026600.15005303739776718No Hit
TTAATCCTTG2905580.1440530973376741No Hit
GGTAACCTTG2900420.14379727440997553No Hit
GGCAACAAGC2869910.1422846469828276No Hit
TTCCACAAGC2791370.13839078404843896No Hit
TTAAACATTG2488800.12338994233647095No Hit
GTCGACAATC2380460.11801865241653633No Hit
GTCAACAATC2338460.11593637277247826No Hit
GGCGGGACGC2224140.11026860589369919No Hit
GGTAGCCTGG2216660.10989776180470978No Hit
GGCGCCACGC2203890.1092646496367426No Hit
TTAATCATTG2065700.10241345382692384No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)