FastQCFastQC Report
Mon 3 Apr 2023
HW22CDRX2_l02.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22CDRX2_l02.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201702015
Sequences flagged as poor quality0
Sequence length10
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGTTC6580230232.62352237780074No Hit
TGCGCGGTTT5843801328.972448787881472No Hit
GGCGCGGTTT3720093918.443513814177813No Hit
GGCAATGTTC104705695.191107783429928No Hit
GGCGCGGGTT51803842.568335274191485No Hit
TGCAATGTTA25146401.246710400984343No Hit
GGCGCGGTGT22485791.1148024475610716No Hit
TGCGCGGTGT18740210.9291037573422358No Hit
GGCAATGGTC18070660.8959087493498764No Hit
TGCAATGGTC16744080.8301394510114338No Hit
GGCGCGGGGT16291740.8077132992449282No Hit
TGCGCGGGTT11621540.576173718443021No Hit
GGGGGGGGGG10461950.51868346481318No Hit
TGCAATGTTT6983480.3462275773496859No Hit
TGCGATGTTT6734320.33387470125174507No Hit
TGCGAGGTTT4972360.24652009549830226No Hit
TGCGCTGTTT4821210.2390263676840313No Hit
GGGGGGGGGT4419920.21913117724679151No Hit
TGCGCGGTTA3788210.18781220405755492No Hit
TGCGCGGTTC3735960.1852217490241731No Hit
TGCGCGGGGT2365790.11729134188371891No Hit
TGCATGGTGA2344050.11621351427748502No Hit
GGCGCGGTTC2313560.11470187841207237No Hit
GGCAATGTTA2230680.1105928465811311No Hit
TGCAATGTGC2154840.10683284448100333No Hit
TGCACTGTTC2108880.1045542356133626No Hit
GGCGCGGTTA2031770.10073126934304548No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)