FastQCFastQC Report
Mon 3 Apr 2023
HW22CDRX2_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW22CDRX2_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232953567
Sequences flagged as poor quality0
Sequence length10
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAACTGT4972062921.34357916914833No Hit
GACGTTCTGT3343398314.352209082078577No Hit
CGGTAACACT2634091811.307368390714533No Hit
GGGTTTCACT147828146.345819980511395No Hit
GGCGGGGGGG126500255.430277442371166No Hit
AACGAACTGT122084675.240729797453585No Hit
GGGTGGGCCG95948404.118777885036635No Hit
GACGAACTGT55445222.380097489556792No Hit
CCCGAACTGT45912921.9709043562316433No Hit
GGGGGGGGGG44577991.9135998033462178No Hit
AACTAACACT41074401.7632011618864802No Hit
AGGTAACACT35253061.5133084439956226No Hit
GGGTAACACT34910051.4985840504429795No Hit
AACTAACTGT24003421.0303950400553428No Hit
CGGTCACACT23862221.0243337463040436No Hit
GACGGGGTGG22467430.9644595826257513No Hit
GACGGGGTGT22377310.960591000523293No Hit
GGGTCTCACT21029740.9027438502369015No Hit
GACGGGGGGG18689370.8022787648493058No Hit
GACTAACACT18183330.7805559809264478No Hit
CACTAACACT11295200.4848691584962938No Hit
GGGTGGGACG11191250.480406895851481No Hit
TACGAACTGT10865240.46641226146152975No Hit
GACGGGCTGT8189890.351567486408139No Hit
CACTAACTGT7650090.32839548664219426No Hit
AACTAACAGT7349400.3154877641345582No Hit
CCCGTTCTGT7329150.3146184921907635No Hit
GGGTGGGACT7104580.30497837365160413No Hit
GGGTTTCCCT6832800.29331167099064No Hit
GACGTTCTGG6103180.2619912662680971No Hit
GACGGGGGGT5993550.25728517820892605No Hit
GGGGGGGCCG5963520.25599608011153574No Hit
TGGTAACACT5783850.24828338430207425No Hit
GACGTTCGGT5619250.24121759852683433No Hit
CCCGCCCTGT5399060.23176550028959206No Hit
GACTAACTGT4886040.2097430858399348No Hit
GACGCCCTGT4763840.2044974052704675No Hit
AGCTAACACT4521100.19407730296741926No Hit
GGGTTTCACG4478000.19222714885494754No Hit
GGGTCACACT4427310.19005117873983876No Hit
GACGGACTGT4037350.17331136208787906No Hit
GACGATCTGT3855780.16551710496023442No Hit
GGGTGGCACT3778970.16221988135515433No Hit
GGCTAACACT3764630.16160430803791898No Hit
GGGTCCCACT3647420.15657283324620652No Hit
GGGTGGGCCT3598530.1544741317483239No Hit
CGGTCCCACT3598160.15445824875478298No Hit
GGCGGGGGGT3474460.1491481776709605No Hit
CACGAACGGT3207640.13769439297746403No Hit
GACTTTCTGT3204950.13757891932172045No Hit
GACTTTCACT2711510.11639701571944594No Hit
GACGTTGTGT2700880.11594070160771566No Hit
CGCTAACACT2463640.10575669785730303No Hit
GACGCTCTGT2392760.10271403141897371No Hit
CGGTCTCACT2344320.10063464707539765No Hit
GGGTGACACT2331540.10008603989309166No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)