FastQCFastQC Report
Mon 28 Nov 2022
HLJKYDRX2_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLJKYDRX2_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439353965
Sequences flagged as poor quality0
Sequence length6
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG15085820834.3363711307351No Hit
CGATGT6997921615.927753377621162No Hit
TAGCTT225680035.136633511433088No Hit
CCGTCC181226024.124829509618742No Hit
ACTTGA167383243.8097582663217797No Hit
ACTGAT155087833.5299062340315968No Hit
GCCAAT117659322.6780074694443696No Hit
TGACCA95064892.1637426215101985No Hit
AGTTCC92514452.105692843809888No Hit
TTAGGC85821901.9533657787747516No Hit
GTCCGC84455881.9222742191481075No Hit
GTGAAA84365711.9202218876071826No Hit
CAGATC75273101.71326779764011No Hit
GATCAG74918941.7052068711841488No Hit
ATCACG72351351.6467667476268253No Hit
AGTCAA70137721.596382998387189No Hit
ATTCCT69280931.5768818656274104No Hit
CTTGTA58125111.3229676896167308No Hit
ATGTCA54093551.2312065967129715No Hit
ACAGTG53584471.2196195839498114No Hit
GGATGT29143760.6633321267511493No Hit
GGCTAC26214520.5966605991594954No Hit
GGGNGG5061640.11520642587122208No Hit
GTAGGC5005590.11393068911987626No Hit
GGGGGT4652540.1058950270313368No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)