FastQCFastQC Report
Mon 16 Sep 2024
HFHGFDRX5_n02_YTW274_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW274_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4705902
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA239232050.83658775724611No Hit
CGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAA1564883.325356116638213No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC1461883.106482030437523No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT1355212.879809226796478No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT1073752.28170922386399No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG871431.8517810188142465No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA803111.7066016249382157No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG737571.5673297063984757No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA493781.049278119263852No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG429370.9124074406989351No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT391880.8327415233041402No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG269160.5719626120560947No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT226880.4821179871574036No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA221780.4712805324037772No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC217500.46218557037524366No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA214950.45676684299843046No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG214310.4554068486764068No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT167870.35672226068456164No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT164650.34987978925187985No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG163650.34775479812371785No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT155770.33100986803380095No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT142590.3030024849646253No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA141040.29970874871597414No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG138100.2934612747991777No Hit
CATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCAT133200.2830488182711837No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA131050.27848008734563534No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC123360.26213890557006925No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT121410.2579951728701533No Hit
CTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGC120040.2550839350245713No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA110780.23540651717779076No Hit
ATTAACCCTAGAAGGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA100920.21445410465411305No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA96400.20484914475482063No Hit
ATTAACCCTAGAAAGATAGTCCGCGTAAAATTGACGCATGCATTCTTGAA92890.19739042589497188No Hit
AATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAG90430.19216294771969328No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG87870.18672297043159844No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG83460.17735175955640384No Hit
CCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC79780.16953179220476755No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAAAATGACGCATGCATTCTTGAA79140.16817179788274383No Hit
CAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGAT71700.15236186388921827No Hit
ATTAACCCTAGAAAGGTAGTCTGCGTAAAATTGACGCATGCATTCTTGAA70650.15013062320464812No Hit
ATTAACCCTAGGAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA70590.1500031237369584No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC70140.1490468777292855No Hit
ATTAACCCTAGAAAGATAGCCTGCGTAAAATTGACGCATGCATTCTTGAA65950.14014316490228654No Hit
CAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTA63850.13568068353314625No Hit
ATTAGCCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA63240.1343844389449674No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA62340.13247194692962155No Hit
ATTAACCCTAGAGAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA60680.1289444616568726No Hit
ATTAACCCTAGAAAGACAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59970.12743571795587752No Hit
ACTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59830.12713821919793486No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAGAATTGACGCATGCATTCTTGAA59080.1255444758518133No Hit
ATTAACCCCAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59050.12548072611796846No Hit
ATTAACCCTAGAAAGATGGTCTGCGTAAAATTGACGCATGCATTCTTGAA57940.1231219859657086No Hit
ATTAACCCTGGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA57590.12237823907085188No Hit
AATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAA52310.1111582859141563No Hit
AAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAA52110.1107332876885239No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAGATTGACGCATGCATTCTTGAA51190.10877829585061481No Hit
ATTAACCCTAGAAAGATAGTCTGTGTAAAATTGACGCATGCATTCTTGAA50950.10826829797985593No Hit
ATTGACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA48790.10367831714302592No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAATTGACGCATGCATTCTTGAAA48580.10323206900611188No Hit
AAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATA48170.10236082264356546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)