FastQCFastQC Report
Mon 16 Sep 2024
HFHGFDRX5_n02_YTW272_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW272_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19843856
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA203174710.238670347134146No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA9791314.934177107513782No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA7645543.8528499702880326No Hit
TGAAACTCAAACTGGGAGGAAGATAAGAAAAGCTTTAACCCTAGAAAGAT6456943.253873642300166No Hit
GCTGTGAATGCTGCGGGCATTCTCCTCCGCAGAGCTCTTTCTTAACCCTA3189581.6073388156011614No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT2729341.37540808600909No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA2304241.1611856082809713No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT2256241.1369967611133642No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA2198871.108086049404914No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG2076521.046429685843316No Hit
TAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAG2022221.0190660524849606No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA1847160.9308473111274341No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT1740110.8769011426005107No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT1689930.8516137186240416No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT1543520.7778326954196805No Hit
TCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATC1452180.7318033349969885No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG1400130.7055735538496147No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA1354760.6827100539330663No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC1169130.5891647268555064No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG1142950.5759717264628407No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT1059850.5340947848039211No Hit
ACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATAT900850.4539692285612231No Hit
TGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGG867610.4372184518976554No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT817820.41212756230442305No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA759330.38265244416206207No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG737560.37168179410292035No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC719320.36249003217922965No Hit
ATTGAAGATTGTAAATCTAAATCCAAATTATGAATGATTTTCTTGGCCTC622580.3137394264501818No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC596080.3003851670763989No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG595560.3001231212320831No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG568360.28641610783710586No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA506380.25518225893193336No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA496910.25041000095949095No Hit
TTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA461070.23234899507434442No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC442900.2231925085527732No Hit
TAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGA308210.15531759553183616No Hit
CTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA248670.1253133463576837No Hit
TTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCA208090.10486369181473601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)