FastQCFastQC Report
Mon 16 Sep 2024
HFHGFDRX5_n02_YTW268_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW268_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5001122
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA165772733.14710179035824No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA1611173.2216170691296875No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT1253672.5067774791336825No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT1030382.060297669203031No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC907041.8136730117761575No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT832381.6643865116667818No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA543311.0863762171768656No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG435840.8714844388919127No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG428580.8569676964489169No Hit
TGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGTCG409960.819736051230104No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT358980.7177989259210233No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG314750.6293587718915875No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC300950.6017649639420913No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT295440.5907474362752998No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG274680.5492367512730144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214100.4281039334773277No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA202850.40560898134458623No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT201140.4021897486204096No Hit
ACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATT195120.39015244979026703No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT193270.386453279883994No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA179850.35961930142875936No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG172340.34460267116059157No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA163020.32596685303817824No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG156410.31274981894062975No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT154570.30907064454736355No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG151450.30283204448921663No Hit
CTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA151390.3027120714111753No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA137480.2748983128186035No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT131730.2634008928396468No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT105110.2101728372153289No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA104670.2092930346430261No Hit
TATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTC103670.20729348334233796No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA102420.20479404421647782No Hit
GCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCT95430.19081718062466782No Hit
TGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGCATGAG87290.17454083303706647No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC84440.16884211183010533No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC81070.16210362394678635No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA78980.15792456172834818No Hit
GAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCT77240.15444534246515082No Hit
GACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCA73670.14730694432169422No Hit
CTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGA73590.14714698021763917No Hit
CCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC72530.14502745583890975No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG72410.14478750968282716No Hit
GCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAG69770.13950869424901052No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA69220.13840894103363205No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA65340.13065068198696211No Hit
CTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGC64120.1282112294001226No Hit
GCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTG63610.12719145823677167No Hit
GATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGA62820.12561181270922805No Hit
ATTAACCCTAGAAGGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA61940.1238522075646225No Hit
GCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGT59470.11891331585192283No Hit
GGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAAC58520.1170137421162691No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT56480.11293465746286532No Hit
ATTAACCCTAGAAAGATAGTCCGCGTAAAATTGACGCATGCATTCTTGAA55310.11059518244106023No Hit
GTAGGTCAGGTTGCTTTCTCAGGTATAGCATGAGGTCGCTCTTATTGACC55270.11051520038903269No Hit
TGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCC55240.11045521385001206No Hit
CATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCAT54930.10983535294679873No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG54360.10869560870540651No Hit
TAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGT54070.10811573882820696No Hit
ATTAACCCTAGGAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA52440.10485647020808529No Hit
CGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATT52230.1044365644349408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)