FastQCFastQC Report
Mon 16 Sep 2024
HFHGFDRX5_n02_YTW257_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW257_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14327759
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA293511720.485527429655956No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT170299311.885969047916007No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG149348010.423681749532498No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT3593902.5083476069076815No Hit
GCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG2754121.9222266371175003No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA2689641.8772230884118026No Hit
GTGCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT2453281.7122566062145517No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT1631881.138963881232229No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC1425460.9948938979222083No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT1420220.9912366616440157No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA1246020.8696544937697513No Hit
CTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA1019420.7114999631135616No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG813170.5675486305988257No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT671650.4687753332534418No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG666650.4652856039803573No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT637110.444668283434974No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA597820.417245990807076No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA492370.34364760043772374No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG490260.34217493468448207No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA418530.2921112785328117No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA411980.28753973318507103No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG408040.28478982651788043No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC390230.2723594108471534No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT389890.2721221092565837No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA380170.2653380755497074No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG337470.23553578755756568No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT319210.2227912962522611No Hit
GCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGT305240.21304099266326298No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG260780.18201031996699552No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA251460.175505464601966No Hit
GCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCT246690.17217626287544338No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA245820.17156904998192668No Hit
CTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGA221560.15463688354892066No Hit
TAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAG220940.1542041571190582No Hit
CTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGG214670.1498280366106102No Hit
GCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTG214200.1495000020589403No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT209390.14614288249823298No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC203540.1420598992487241No Hit
TGCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATC196040.13682530533909734No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA185470.1294480176557967No Hit
GCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAG182330.12725646767229964No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT177690.1240179989068772No Hit
GGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAAC177590.12394820432141553No Hit
GACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCA168600.11767367108840958No Hit
ACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATT159960.11164341890451954No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT158500.11062441795677888No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA157110.10965427321886138No Hit
TATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTC155460.1085026625587435No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC147590.10300982868290846No Hit
GATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGA146970.10257710225304599No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG145980.10188613585697527No Hit
GAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAA143460.10012731230334065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)