FastQCFastQC Report
Mon 16 Sep 2024
HFHGFDRX5_n01_YTW274_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n01_YTW274_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4705902
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC376468879.99928600298094No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTAGATCGGAAGAGC1469613.1229082118582157No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACAGATCGGAAG745081.5832883897709729No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTAGA504071.0711442779726394No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATAGATCGGAAGAGCAC393890.8370127554717459No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGAGATCG215750.4584668359009601No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATA181160.38496339277783514No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACACAGACTATC158580.33698109310393626No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACAGAT157120.3338786060568197No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCGGACTATC156560.33268861102504893No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGGCTATC144050.30610497201174186No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTAGATCGGAAGAGCA138720.29477876929863817No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGAGATCGGAA131910.2803075797158547No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCATTTTTACGCAGACTATC117380.24943145862366023No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTACC116770.24813521403548142No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACCATC97150.2064428881009422No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTGTC94930.20172540779642245No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTCTACGCAGACTATC92860.197326676161127No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTAGATCGGAAGAG84060.17862675423330107No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTATGCAGACTATC79640.16923429344682486No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTACGCAGACTATCT76040.1615843253854415No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCTTACGCAGACTATC71230.1513631180589821No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTGCGCAGACTATC68960.1465393881980543No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTCACGCAGACTATC51280.10896954505214941No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCATACTATC50690.10771580028653381No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCTCAATTTTACGCAGACTATCT49900.10603705729528579No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGCAATTTTACGCAGACTATCT49310.10478331252967019No Hit
GTCGTAAGCAATATTTCAAGATGCATGCGTCAATTTTACGCAGACTATCT48980.10408206545737672No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCATCAATTTTACGCAGACTATC48770.10363581732046269No Hit
GTCGTAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCT48300.10263707149022652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)