FastQCFastQC Report
Mon 16 Sep 2024
HFHGFDRX5_n01_YTW268_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n01_YTW268_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5001122
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC374524274.88803512491798No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGATCGGA1622233.243732106515298No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTAGATCGGAAGAGC919431.8384474523916832No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTAGA552181.1041122372139691No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAAGATCGGAAGAGCACAC412680.8251748307679756No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACAGAT365200.7302361350113035No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACAGATCGGAAG317400.634657582838411No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATAGATCGGAAGAGCAC299420.5987056504520386No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATATCGGAAGAGCACAC195980.3918720639088588No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTAGATCGGAAGAGCA157170.31426947792915266No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGATCGGAA152590.3051115329720011No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGAGATCG150240.300412587415384No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGAGATCGGAA139930.2797972135052894No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCGGACTATC128630.2572022838075136No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGGCTATC122110.24416520932702704No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTACC110060.2200706161537351No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCATTTTTACGCAGACTATC108580.21711128022871667No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATA99610.19917530506154418No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACCATC95330.190617225494599No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTCTACGCAGACTATC85660.17128156441694484No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTGTC80100.16016405918511886No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTAGATCGGAAGAG76910.15378549053592375No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACACAGACTATC76030.1520258853913182No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTATGCAGACTATC66790.1335500313729599No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTGCGCAGACTATC65300.1305706999349346No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTACGCAGACTATCT60280.12053295240548022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)