FastQCFastQC Report
Mon 28 Oct 2024
HFHGFDRX5_n02_YTW274_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW274_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4700243
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA239187050.88822003458119No Hit
CGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAA1564853.3292959534219824No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC1461723.1098817656874336No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT1354802.882404165061253No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT1073002.2828606946491914No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG870681.8524148645080691No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA802351.7070394020053856No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG737431.568918883555595No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA493601.0501584705301408No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG429230.9132081043469454No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT391680.8333186177821019No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG269080.5724810398100694No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT226690.48229421329918476No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA221690.4716564654210431No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC217370.4624654512543288No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA214910.4572316792982831No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG214130.455572190629293No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT167840.3570879207734579No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT164610.3502159356441784No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG163610.3480883860685501No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT155610.3310679894635235No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT142570.30332474299733014No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA141030.3000483166508625No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG138060.2937294944112464No Hit
CATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCAT133190.2833683279779365No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA130990.27868771891155414No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC123310.2623481381707286No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT121330.2581355900109845No Hit
CTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGC120030.2553697755626677No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA110670.23545591153478662No Hit
ATTAACCCTAGAAGGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA100910.21469102767665416No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA95730.20367032087489945No Hit
ATTAACCCTAGAAAGATAGTCCGCGTAAAATTGACGCATGCATTCTTGAA92880.19760680458435873No Hit
AATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAG90430.19239430812406932No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG87830.18686267922743569No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG83270.17716105316257053No Hit
CCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC79760.16969335415211512No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAAAATGACGCATGCATTCTTGAA79120.168331722423713No Hit
CAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGAT71700.15254530457255083No Hit
ATTAACCCTAGAAAGGTAGTCTGCGTAAAATTGACGCATGCATTCTTGAA70630.1502688265266285No Hit
ATTAACCCTAGGAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA70590.1501837245436034No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC70090.1491199497557892No Hit
ATTAACCCTAGAAAGATAGCCTGCGTAAAATTGACGCATGCATTCTTGAA65940.14029061901693168No Hit
CAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTA63840.1358227649081122No Hit
ATTAGCCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA63230.13452495966697892No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA62290.1325250630658883No Hit
ATTAACCCTAGAGAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA60670.12907843275337041No Hit
ATTAACCCTAGAAAGACAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59970.12758914805043056No Hit
ACTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59830.12729129110984264No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAGAATTGACGCATGCATTCTTGAA59080.1256956289281214No Hit
ATTAACCCCAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59040.12561052694509625No Hit
ATTAACCCTAGAAAGATGGTCTGCGTAAAATTGACGCATGCATTCTTGAA57940.12327022241190508No Hit
ATTAACCCTGGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA57590.12252558006043517No Hit
AATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAA52300.11127084280536134No Hit
AAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAA52110.11086660838599195No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAGATTGACGCATGCATTCTTGAA51180.10888798728065763No Hit
ATTAACCCTAGAAAGATAGTCTGTGTAAAATTGACGCATGCATTCTTGAA50940.10837737538250683No Hit
ATTGACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA48780.10378186829914963No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAATTGACGCATGCATTCTTGAAA48580.10335635838402397No Hit
AAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATA48160.10246278756226009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)