FastQCFastQC Report
Mon 28 Oct 2024
HFHGFDRX5_n02_YTW257_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW257_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14320011
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA293458920.492924202362694No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT170281011.891122150674326No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG149334010.428343944707864No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT3593332.50930673167779No Hit
GCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG2753971.9231619305320364No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA2689261.8779734177578495No Hit
GTGCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT2452991.7129805277384214No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT1631611.1393915828695942No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC1425270.9952995147838922No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT1419920.9915634841341953No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA1245620.8698457005375205No Hit
CTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA1019330.711822078907621No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG812980.5677230275870598No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT671600.4689940531470262No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG666470.4654116536642325No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT636860.4447342952460022No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA596950.41686420492274767No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA492040.3436030880143877No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG490210.34232515603514546No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA418500.2922483788594855No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA411910.2876464270872418No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG407970.28489503255269844No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC390170.27246487450323886No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT389800.2722064948134467No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA379970.26534197494680695No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG337240.2355026123932447No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT319200.22290485670716315No Hit
GCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGT305230.21314927760879512No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG260660.1820249998411314No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA251390.17555154112660948No Hit
GCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCT246630.1722275213336079No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA245730.17159903019627568No Hit
CTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGA221540.15470658507175727No Hit
TAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAG220720.15413395981329903No Hit
CTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGG214670.14990910272345462No Hit
GCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTG214170.14955994098049227No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT209350.14619402177833524No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC203460.14208089644623878No Hit
TGCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATC196020.13688536971095902No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA184750.12901526402458768No Hit
GCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAG182300.12730437148407217No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT177680.1240781169791001No Hit
GGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAAC177590.12401526786536686No Hit
GACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCA168570.11771639002232609No Hit
ACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATT159910.11166890863421822No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT158290.11053762458702021No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA157110.10971360287362908No Hit
TATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTC155430.10854041941727559No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC147500.10300271417389274No Hit
GATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGA146940.10261165302177491No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG145900.10188539659641324No Hit
GAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAA143440.10016752082103847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)