FastQCFastQC Report
Mon 28 Oct 2024
HFHGFDRX5_n01_YTW274_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n01_YTW274_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4700243
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC376158980.02967080638172No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTAGATCGGAAGAGC1469443.126306448411284No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACAGATCGGAAG744941.5848967808685634No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTAGA503881.0720296801676No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATAGATCGGAAGAGCAC393670.8375524414376022No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGAGATCG215570.4586358620181977No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATA180970.38502264670145775No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACACAGACTATC158530.3372804342243582No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACAGAT156970.333961456886378No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCGGACTATC156530.33302533507310156No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGGCTATC143990.30634586339472236No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTAGATCGGAAGAGCA138700.29509112613964855No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGAGATCGGAA131850.2805174115465945No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCATTTTTACGCAGACTATC117370.24971049369149637No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTACC116710.24830631097158168No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACCATC97100.20658506379351024No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTGTC94920.20194700571864052No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTCTACGCAGACTATC92850.19754297809708984No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTAGATCGGAAGAG83840.17837375642067868No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTATGCAGACTATC79630.16941677270728345No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTACGCAGACTATCT76030.16175759423502145No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCTTACGCAGACTATC71220.15152408077624924No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTGCGCAGACTATC68960.14671581873532924No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTCACGCAGACTATC51280.10910074223822044No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCATACTATC50650.10776038600557461No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCTCAATTTTACGCAGACTATCT49880.1061221728323408No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGCAATTTTACGCAGACTATCT49310.10490946957423265No Hit
GTCGTAAGCAATATTTCAAGATGCATGCGTCAATTTTACGCAGACTATCT48940.10412227623125019No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCATCAATTTTACGCAGACTATC48720.10365421532461194No Hit
GTCGTAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCT48270.1026968180155792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)