FastQCFastQC Report
Mon 28 Oct 2024
HFHGFDRX5_n01_YTW257_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n01_YTW257_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14320011
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATC1102411576.98398416034736No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGCAGATCG2775911.9384831478132243No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGATCGGA2702131.8869608410217003No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGCACAGAT2474381.7279176671023508No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTAGATCGGAAGAGC1459981.0195383229803385No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACAGAT1432851.0005928068072014No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTAGA1269530.88654261508598No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGATCGGAA1023960.7150553166474523No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGAGATCG820300.5728347555040286No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACAGATCGGAAG686890.4796714192468148No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGAGCTGAGTA649020.4532259088348466No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATAGATCGGAAGAGCAC646440.45142423424116085No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATA494920.34561425965385084No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCGGACTATC379260.26484616527180044No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGGCTATC318470.22239508056243812No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTACC299800.20935738108022406No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTAGATCGGAAGAG273500.1909914734004045No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGAGATCGGAA258920.18080991697562243No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACCATC237300.16571216320993049No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTGTC228280.15941328536688973No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTAAG223830.15630574585452484No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGAGATCGG215580.1505445770956461No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTACACAGACTATC203990.14245100789377885No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGCAAGATC197970.13824710050851216No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATCGGAAGAGCACACGT172980.12079599659525402No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTTTGCGCAGACTATC158680.11080997074653086No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCATTTTTACGCAGACTATC157310.10985326757081403No Hit
GTCGTAAGCAATATTTCAAGAATGCATGCGTCAATTAGATCGGAAGAGCA146220.10210886011190914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)