FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MTH-040_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-040_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGCCTTGACTTCAGAGAGGCTTTTCCGAGGAAAACTGCAAGAAGTGA16.25No Hit
CTCCAAGCTTCACATTTTGTGTATTGGTTCAGTTTTTTTTTTAAAGTCAG16.25No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA16.25No Hit
TCATAAATAAATAAAAAATAAACCTAATAATAAGAAACGCAATAAGCATA16.25No Hit
AAGAGAGAAAAAAAGTCAAGCGGGACACTGATTCTTCGGATTTATGTTGC16.25No Hit
TAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAAA16.25No Hit
TTTCCATCAACTTCAAAGATCCCTGGTGCCATTTGTAGTCCTCCAACCCA16.25No Hit
GCATATTTTATATGTAGAAACTGCGAACGGCTCATTAAAACAGTTATAAT16.25No Hit
CAGCAAGAATGGAAAATTTTGCACCTGAGTTTCAGGTAGGAGATGGAATT16.25No Hit
ACAAGGAAGTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTA16.25No Hit
TTTTGAAGATATATCTGCAAAGAAGAAAAAAAGCCAATGCCGTCGTGGAG16.25No Hit
AATGATTTCAACAAGGATATTTGTATCAGGGTACTACTTCACTTCATTAT16.25No Hit
ATTGATGGGGAAATATACTACTCGGTTCGCCGGGTATTCATATTAATTGA16.25No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG16.25No Hit
AAGGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTA16.25No Hit
TTTAAATGACGTTTTCCACCACCAAGGAAACAGCCACCTAGCCAACGGTA16.25No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)