FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MTH-003_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MTH-003_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGAGTAT15.0No Hit
GTATTGTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGAC15.0No Hit
GGGGAACCTGAAGAACAAGACCCTCACCTTACCAAGAACCTCCGTCCTAA15.0No Hit
CGATATTTTAAGCAACGCTTTTAGCTTAATCCACATAACTGATACTTCGT15.0No Hit
CGGCGAGTGAACAAGGAGCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA15.0No Hit
GAAAAATACCAGAGACAAAGTGAACCCTTTAATTTCTCTAAATTTGGCTA15.0No Hit
AACTGATTGTACTTATGCATTTTGTACCAGTGGAACTTTTTATACTGGAG15.0No Hit
CGAAAGATGGGGAACTATGCCTGGTCATGGCGACGCACGAGGACACTCTG15.0No Hit
CGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTA15.0No Hit
GTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCGATCTATTATTG15.0No Hit
TGGTCATGCCACTAATTCGTTCTCTTCTGTGCAACAGATTGAGACCTTCT15.0No Hit
CGCAAAACCGCCGAATTTAAGCATATAATAAAGCCAAAGAAAACAAAAAC15.0No Hit
GTTTGAGACTAGCCTGGCAAACATGATGAAACCCTGTCTCTACTAAAAAT15.0No Hit
ACCATTAAAGAGCTATGAAAAGAAGCTGGCAGGGGTAAGATGAAGGGGTA15.0No Hit
GAAGACTGCGATGACTTCTGCGCTGACCCAGGGGCTGGAGCGAATCCCAG15.0No Hit
GAAGAAGGGCTCCACCATGGAGCGCTGGGGACAGAAGCACATGACCGCCG15.0No Hit
GAGAAGATGAATCTCGGCGTGCCAGAAGCGAGCACAGAAAGTGAGGCAAA15.0No Hit
CAAATGAGAGCATCTATAACAGTAGACAAGTAACTAATCTACTCAACAGG15.0No Hit
GTTTAAGCTCTTATTCCAGTCCTATAATGTGAATGATTGGCTTGTCAAGA15.0No Hit
ATTGAAGTCAGACGAAAGTCTGATGGAAGATCGAATTGATACTGACGTGC15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)