FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-091_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-091_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTA11.5873015873015872No Hit
AATGAAAATCATATAAATTGTAAAAATAATAAAAAAAAATAAAATGAAGT11.5873015873015872No Hit
CACCAATAAAGAAATAAAAAAAAAAAAATCACAAATTTTAAAATCGACTA11.5873015873015872No Hit
GACAAACAAGACAAAGCCCAGCGGCGACAGCACACACGCCACAACATGTC11.5873015873015872No Hit
GACGGGGGGGCCTGGTGGGGTTGCGCGCACGCGCGCACCGGCCGGGCCCC11.5873015873015872No Hit
CGAAATACAGTAATTATCTTAACAAAAACCTAATCAATTCTCAAATTTAA11.5873015873015872No Hit
CGGGCATCAATTAATTAGGAAAAAATAAGCTTTGTCTTATGATACAAACT11.5873015873015872No Hit
CAGAGTTCCTGAACTGAATGTTCAAAATGGAGTGTTAAAATCGCTTTCCT11.5873015873015872No Hit
GGGGAGGCTAAGGTGGGAGGATCCCTTGAGCCCAGAAGGTTGAGGCAGTG11.5873015873015872No Hit
GCGAAACCAGATTCAAACAAATATAAATATAACAAGCAACCAAAATAAAA11.5873015873015872No Hit
GTTATAGAGTGAGGTGTAGCATATTGATGATTGATGTTCATGTTGGGAGA11.5873015873015872No Hit
GGGGAAGTAGGTTAAAAATCACATCAAAAAGCTAATAAAAGGACTGGTGT11.5873015873015872No Hit
CTGGTATTCTCAGGCTGACAGAGATTTGAATTCTCCCTGAGATGGAGTGC11.5873015873015872No Hit
CAGAAGCCTCTGGGACACCAACATTAGGGGATGGCACCGAGAGAGGCCTG11.5873015873015872No Hit
ATGTAACAAAAATACAAATCAACTCCTTAACAAAAGTATTACAATACGGG11.5873015873015872No Hit
AACAAACCTTGGAATCAATTTACTTGGAGAAGATGTTCGTTGAACTCAAT11.5873015873015872No Hit
GAACATTTAATTCACAAATGTGATAGAACAAGAAATAGAATCTCCTAATA11.5873015873015872No Hit
CCTTAATGATGATTTAAAAGTCCTTATCAATTCAGATTAACCAGAGGCAG11.5873015873015872No Hit
CATTGAGTCTGGGAGGTTGAGGCTGCAGGGAGCTGAGATCATGCCATTGT11.5873015873015872No Hit
ATTTGCAGACTAGAATTCTAGTGCTGCTGAAGATGAATCAATGGGAAATA11.5873015873015872No Hit
CAAACGACCGTAGTAATAATCAAACTAATAATACCAAATTAACATCTACA11.5873015873015872No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCTTTACCTTCTA11.5873015873015872No Hit
TTCTCAACGTACTTTACAATCATAACTACAATAACTTTAAAAATCTATAA11.5873015873015872No Hit
AAACACGGGTGCCGGGCACCCCCCTGGTTCCAAGCGGCACCACCAACTGC11.5873015873015872No Hit
GTTATCTTGAAGGATATGGAAAGCTTTGCCACAAAAGTACTACAAGACTT11.5873015873015872No Hit
GGTAAAATTTAATCAAATAAGTTAAGCATATCAAATATTGGGAGATATTG11.5873015873015872No Hit
CCCAGTTGTAAAGTATGCTGAGAAGTATCATCAATTTTGAAGTCAAATAC11.5873015873015872No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGAGGGAGTAGAGACTGAAAAA11.5873015873015872No Hit
GAAACTACAAAATAATAAACATTTTCAAAGTCTTTAACAAAATTACTCAC11.5873015873015872No Hit
AAATATTGGGAAACGTAACAGTTTCCCTTTCCCTTTTCTACTTAGTTCGC11.5873015873015872No Hit
CTCAACAAAGAAGAAAACAGTACCATAAGCAAGCCAGAACCACCAAACTG11.5873015873015872No Hit
GGGTTCTAGTTCAATTTGTTTAGTATAAATTGTCATAGCTGGTTTACTGA11.5873015873015872No Hit
CACAAATTTATTAAAAGAAAAACCTAAATATAAATAAAACAATGACATTA11.5873015873015872No Hit
AAAAAAAATAAAAATGGACCAAATAAAAAAACAATTAATATATAATTTAA11.5873015873015872No Hit
GCAACACTGGGCAAACAGACCTACAAGAGAAGCGAAGTCGTAGGACCCAC11.5873015873015872No Hit
CGGTGGGCGGCGGCGGGAGGGTTTGGGGGGTGGGGTGGGGGTGGGGGGGG11.5873015873015872No Hit
GATAAAATTAAAATACAAAAAGAAAAGAGAAAGTAAAGCAATATTAGAAG11.5873015873015872No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC11.5873015873015872No Hit
AGTTACAAGGAAATATCTAACTAATATGTTGAAAGGACCGTAGTTTATAC11.5873015873015872No Hit
ATCATTTCACTCACGAATATGCGCGAAAGACTAAACTAAAAGCCTATTAG11.5873015873015872No Hit
TTATGACATAACGCCACTAAGTCAGACTCCCGCCCAGCAGGAACGATACG11.5873015873015872No Hit
CCCTAAGACTCTAGTCATAACATCACGTGTCACAGGAAGAGTGAGTAATC11.5873015873015872No Hit
TCCCCCACATGAGGACTTAATGGTACAGATACATTCTATTCTTGCAAAGA11.5873015873015872No Hit
TTAAACCTTTCCTTTTCAAACAGATACAGAATATCATAACTACACAAAAC11.5873015873015872No Hit
CCCAATTCTTACCACAAGGCACACATACACCCATTATCCCCATACAAGTT11.5873015873015872No Hit
GGCCTGGCTGTCCTGGCTGTCCTAGCTGTCCTAGGAGCTGTGATGGCTGT11.5873015873015872No Hit
CCTGATTTTAGAGATTTTCTGAGTCTCAGAAGTTAATTAAGTAAACTACA11.5873015873015872No Hit
AAGTAGTGACACGTAATTCAACCCTCCATATTTACAGTGTATCAATCCAG11.5873015873015872No Hit
ATAAAAACAAACATGTACAGAACAAAAACAAAAGATATATAAAAAATAAA11.5873015873015872No Hit
AGAAGAAGGCTGCTGGCAAAGGGGACGTCCCAACGAAGAGACCACCTGTC11.5873015873015872No Hit
GTACAATGATCTGCCCAGTAGTAAACATGGCGCTAGTCTTCTTTTAAAAT11.5873015873015872No Hit
AAAATAACCTTACTAATAAACATTAAAAAAAACTAAAGTTAAAACTAGCC11.5873015873015872No Hit
GATTATCGAAGAATCAATTCATTCAAATTATTTTTTTCTTTTTTCTCAAA11.5873015873015872No Hit
CTTGTCAACTGTGATTTGAAAAGAGATAGAATCTAATAATTTATAGCTTA11.5873015873015872No Hit
TTCCCTTCTCCTGGCAATCCCTTCCGCTTCCCCGGCTCCCGGCGTGACAT11.5873015873015872No Hit
GAAATACAAATTATTTTACCAACTTGGTATTGAATTGTTGCTTTATATCA11.5873015873015872No Hit
ATTCTGTATTTTTAGTCAAACATATTTATTAATTGAATATAGAAGAAAAT11.5873015873015872No Hit
CCACCCCACAACAGGCCCCGGTGTGTGATGTTCCCCTTCCTGTGTCCAAG11.5873015873015872No Hit
TGGAAAAGTCTCCTTGCCAAACAGATGTCCTCCGCAGTGCAGCCAAATTT11.5873015873015872No Hit
GTAATAATGGCCCTCAAGAAAAACCACTGAGATACACTAAATGATAAATT11.5873015873015872No Hit
GTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTG11.5873015873015872No Hit
GAAAGAAAGTGTACAATAGAGTGTATAAGTTATACTCAAATATGAGTCAC11.5873015873015872No Hit
AGATGTAACATCACTGGGTCCAGCCATAGCCTCAGGAAACTCTGGACCTG11.5873015873015872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)