FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-080_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-080_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATAAAATGGACTAAGAGTCTCACAATATAATTCTCTTCACTTAATAT11.694915254237288No Hit
GGCGAAATAACTCAATGTGTATTTCACTCATATTAAATAAAATAACTCCA11.694915254237288No Hit
AATCACCAGCACTGGCTGAAGGGACATTAACATGGGGAATCTTCTTAAAG11.694915254237288No Hit
CAGTCTAACAGTTGTGTGAACAGATTACAGGGTTTTGCGACCTCACCCGG11.694915254237288No Hit
AGGACGCCAACTGCACTGGAGCTGGGCTCCAGAGCTGCAGAGCTAGTGTT11.694915254237288No Hit
CATGTTTAACTTAAGGATAAAGTCAAAGAGCCCAACTATTCTTACAGGGC11.694915254237288No Hit
ATTTACCTCTTGAATGGTGCCTTTTTATGAACAGAAATTCTTAATTTCAA11.694915254237288No Hit
GCTATTGACACTTCCATTTTGTGTGGGAACGTTTCTTCTGGAGAATTGGA11.694915254237288No Hit
GCCAACCCCAACAGACCCGTAGGTGGAGAAAAGGGAGGAAAAAAGTAATT11.694915254237288No Hit
TATAGTTTTAAATCAGATTTACTTTCTTAACTCTGTTCTCTTTTGTTTGT11.694915254237288No Hit
CACGCAATAGACAAAAGCCAACAAATATGATAACCGCAAACTCATAAATG11.694915254237288No Hit
CACATTTCTCAGACAGTATCCCTGACATTGACTGATGCATGACTGCACTA11.694915254237288No Hit
AAAGAAACCAAAACACAAAATGACTTAAAACAAAGAACAGACAAGAAATG11.694915254237288No Hit
CCAGAACCTGTGTCTTTTGGCCAAGTGTTTCCTTGACCATAAGACACTGT11.694915254237288No Hit
CTGTGGACAGGTCTGAAGACATAGAGTTTTGTTCTCCTGTGGCCCCATCA11.694915254237288No Hit
CTATTTTTTGATTTTTTATTATGGCCATTCTAGAAGGATTAAGATGGTAT11.694915254237288No Hit
CCGAAACATCATGGGAAAAAGGAAGCCTGGTGTCCCCGGGAGGCCTGCAG11.694915254237288No Hit
ATTTTTTAGGCGCCTTTTCGAAAACCTAGTAGTTAATATTCATTTGTTTA11.694915254237288No Hit
AATCTTATAAAAAACAGAAAGAAATAATCCTGGAAAAACCATTAGAGAGC11.694915254237288No Hit
GGAATTTAGATCTGTTTGGTACTTCTATGATACCATGCATTTTGTGTTAA11.694915254237288No Hit
GGAGGAAGAGTTCAGGTGGAAAAGGAGGGAGCTACTCTCAGGCTGCGTCC11.694915254237288No Hit
CATGTATATCAAATGATATCTAAAAATTAAAAAGAAAAATTTTAAGTAAT11.694915254237288No Hit
AATAAAAAAAATAATTCAATACATAAAGACAATTTACACAAAACACCCTA11.694915254237288No Hit
CTAGAATTTACTCTCAAAGGTATTTCAAAGGAGATTTCTCATCTCAGATA11.694915254237288No Hit
CACAGATCGACTTTACTAAGCTATTGTGCTTATTACATAAAACAACTCAC11.694915254237288No Hit
TGACAATTCTGCATGTACTAGTCCTCTAGAAATAGGTTAAACTGAAGCAA11.694915254237288No Hit
TTATGGAGCATAACGGGTCCCAGCCAATTGTGATGATACTTTTTGCTAAT11.694915254237288No Hit
GTTTTACCTACAGAGCAATTCAACTCATGCTTATTTATAGTACTAACTTT11.694915254237288No Hit
AGCGGCTTTCCAGGAAGCATGAGTTCATGAGTGACACCAACCTCTCGGAG11.694915254237288No Hit
CGGAAAATAAACATTCAGAGCACAAACACAAAATTGATCAACATGGAAGA11.694915254237288No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTATTGTAACT11.694915254237288No Hit
GCTGGGACTACAGGCAAATTCAACCAAGCCCGGCTAATTTTTTTGTATAT11.694915254237288No Hit
CAAGAAGACAAATTATACCTCCTTCATCTAACCAGGCAGAAATAAAACTA11.694915254237288No Hit
CCCAAGAGCCCAGTGTTCCTGGAGGGCTCCCAAACTCTGGACCTGGAGGG11.694915254237288No Hit
GTGGATGTCATTTAATAACTATAAATTCAAAATAAGCATTGTAAATATCA11.694915254237288No Hit
AAGCCATATTTTCGGACGGCTGTGTAACACTCAAAAAACCCACCCACCAG11.694915254237288No Hit
AGTTAAGCTCTTCCTGGGGACAATGAGGGATACAATGTCAAGAATGTGTC11.694915254237288No Hit
GAAATCGGTACGATGGAACGGATTAAGAGAGAAAACAAGTACAGAGGGGA11.694915254237288No Hit
TGAATATTGGTCCCCACTGTCTTCTGGCTTGTAGAGTTTCTGCCGAGAGA11.694915254237288No Hit
ATGGGCAACTCTTCATCACACAGGTAACTGTTTTCCTGACAGTTAATTTT11.694915254237288No Hit
CAAAAAAGAAGTTTTAAGCTTGTTAGCACAGTTCTTTTTTTCAATTGTAA11.694915254237288No Hit
CTTCATACAGAGAGTCAGCACTGCACTGAGTGAACAGGCTTCATACAGAA11.694915254237288No Hit
CTTAAAAAACTCCCTCCATTAGTAGCTATCCAGATTGAATAGTGTAAATA11.694915254237288No Hit
GCCCAATGGACAGGTCAGTTGGCAGACTCTACAGCTGCAGAACCTCCAAG11.694915254237288No Hit
GAACGTTTGTCCGGTGACCGGCCAAGAAGGAGGCCCGCCTGTTACGAAGG11.694915254237288No Hit
CAACAAAAAAGCTTTTTTCAGCTCTACAGTTAACAACAAACAAAATTCTA11.694915254237288No Hit
AGAATGTGTATTATGTAGAAAATGTCATCAAACAAACTTGGTTATGATAC11.694915254237288No Hit
ATTCTTTAAACATTTCTCTTTTTAATATACGCATAATTAATGTAATATGT11.694915254237288No Hit
AATGAAGAGGCAATGTCCATCTCAAAATACTGCTTTTACAAAAGCAGAAT11.694915254237288No Hit
CTAGAAACATTATATCTGAAAATGAAAGTCTCAGTCAAAGAAAAAAATGA11.694915254237288No Hit
CTTAAATCTGTGCTTACAGAGAGGCCAGCTTCCCTTCTTGTTAACCCATA11.694915254237288No Hit
TATTAAATGTAGAGAGAAATTATCACTTACACGTGTACCTTATTTTGTAG11.694915254237288No Hit
GATCATTTGAGGACATGAGTTTGAGACCAGCCTGGCTAACATGGTGAAAC11.694915254237288No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT11.694915254237288No Hit
TTGGGTGGGGGTACAGCCAAACCATATCAGATAGGTTATTTATATATTCC11.694915254237288No Hit
CACAGATCCTGCAAGAAGGCCCCCCGGTTTGACTTGTATTTGCAACCGCG11.694915254237288No Hit
AATGAAGTAGAAATCGTGGCCCACCTCCCTCTTGGGGTCGTCGCCCTCGA11.694915254237288No Hit
AGATGATACATAACGATAAATAGAAACGGGGAACTAAAGCTGGCAGTGGA11.694915254237288No Hit
CGGCAAGGTATACCCTATTACCAAAGTCCACAATCCATCACAAGTAAGAG11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)