FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-079_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-079_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCACGCGAATGGTCACGCGAATGGTCACGCGACTGGTCACAAACCCTTT18.333333333333332No Hit
CCTACCTTAGTTAGCCTCACAAGTAGCTGGGACTACAGGTGTGCTAACCA18.333333333333332No Hit
TTCCTGGGCATGGAGTCCTGTGGCATCCACGAAACTACCTTCAACTCCAT18.333333333333332No Hit
AAACCATGATAACTGGCAGACTGCAGAAGCTTTTCTCTTGTCACCGGAAC18.333333333333332No Hit
GCTCTGCCTCGGAAGGAAGGAGGTGGGTGGACGGGGGGGCCTGGTGGGGT18.333333333333332No Hit
AGTGTGGACAGAGAAACTGGGGAGGGAGATACCACGTCTGCAGCAGGCTG18.333333333333332No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA18.333333333333332No Hit
AGAGCAACTAGAAAAAAGGAGTGGCACAGGGACAGAAACTTCTTTTCTCC18.333333333333332No Hit
CAAGTTTTGCTTGTTGTGTGCTTGAAAGAAAATATCTCTGACCAACTTCT18.333333333333332No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG18.333333333333332No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC18.333333333333332No Hit
GTCAAGCGGTTAATGACGCCGAATGATTGGCTTTTGTACCCCCGCGTGGG18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)