FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-078_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-078_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA22.3255813953488373No Hit
TGCATTCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGT11.1627906976744187No Hit
CGCAGAAGCCCCAGACCAACAAGTCTGCCTACAACAGCTACAGCTGGGGG11.1627906976744187No Hit
ATCACCCTTGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAA11.1627906976744187No Hit
GGCCGAGAAAGGGAATATGCGGGCGAATCGAACGAGCGCACCGAACGGGC11.1627906976744187No Hit
TGGGGAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAA11.1627906976744187No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA11.1627906976744187No Hit
CGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTTTTTCGTGAGC11.1627906976744187No Hit
CTACTTTTAAAGTTGTTTTACTTATCTATTGATGGGGAAATATATTACTC11.1627906976744187No Hit
TTTGCCAATAAAAAGTCCCAAGGTGACAGTACAAGAGACGCCTTTAGTGA11.1627906976744187No Hit
TGTGTGTCTAACACAAGGAAGTTTAAGGCAACAACAGGTCTGTGATGTCC11.1627906976744187No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG11.1627906976744187No Hit
GATGAGGTAGACATGACTAGTAAACCGGCTAGACATTTTCTGGAAAGCCT11.1627906976744187No Hit
CTACAAACTAAAAAAAAAGAAAGAATAAATAAAAAAAGATAATTAAACAA11.1627906976744187No Hit
GAATGGACAAGTAAAGAAAAACAACCATCAAGAAGATAAAATTGAATTTG11.1627906976744187No Hit
GTTCAGCAAACCACCATGGCACGTGTATACCTATGTAACAAACCTGCACA11.1627906976744187No Hit
TCCCAACCTCCAAGGCTTTTGAAGAACAAAGCTTTATTATGCAAACCCAT11.1627906976744187No Hit
CAGTAAAAAAAAAGAATATAAAATAAAACAAATACAAAAAAATAAAAAAA11.1627906976744187No Hit
GGTGGACCTAGAAGGCTTAGGGTCATTCTTCCTAAGCATCAACAAACATA11.1627906976744187No Hit
TTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTTGGAA11.1627906976744187No Hit
CATGTTCGTGAGCTGTGAGCTGGTAATGTAAATTATTAGTTTGCCGTTTC11.1627906976744187No Hit
CTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAA11.1627906976744187No Hit
GTGCACTTTGGCAGCATAATTGGAAACGAAGAAAATGTGCTGGAAGTCAC11.1627906976744187No Hit
GTGTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATG11.1627906976744187No Hit
CTTAAAAATTTATCTAAAACTATCAATACCAAAAATCAGTAGATACTTCA11.1627906976744187No Hit
GGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGA11.1627906976744187No Hit
ACCTGTCACAGATGAAGAACAGACCAATGTGCCTTACATCTATGCCATTG11.1627906976744187No Hit
ATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACATATATA11.1627906976744187No Hit
AGCATTTCCAAAGGGGCAGTTACAAAAAAAAATGTTCCAGCAGCGTTATC11.1627906976744187No Hit
TGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGC11.1627906976744187No Hit
GGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTTTTTACGACAGTGA11.1627906976744187No Hit
CTTCGAAGAAAATATTGGGATACGTAACAGTTTCCCTTTCCCTTTTCTAC11.1627906976744187No Hit
TATATTCAGTCAGTAAATCATAATCCATTAAATAAAAAAGTTAATAAAAT11.1627906976744187No Hit
TCTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTATTTATGA11.1627906976744187No Hit
TCATAAGACTGAACTAGAAAATCCAAATAACAAAAATCAAAAAAAAAAAA11.1627906976744187No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGAGCATTTTGCT11.1627906976744187No Hit
ACGAAATAGAGTCAAATTTGTGATTGAAAAGAGGGGAAGAAAAGGATTAG11.1627906976744187No Hit
CCCACACTAACGAGGGGACAACTGGTTTTATAACTCTTCTCAAAAGGGGA11.1627906976744187No Hit
CAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGC11.1627906976744187No Hit
CTATATCGTTCTTATTTGTTATCCTTTTACTGATGATGTAAAAGAGAAAT11.1627906976744187No Hit
CTGGTTTTATAACTCTTCTCAAAAGGGGAATTATCTTATTTGCTGAGGAA11.1627906976744187No Hit
CAAAAAATTAAATCAAGCTGCTACATGTCCAGCAGATACAATAAGAATGC11.1627906976744187No Hit
CTGGTTTTATAACTCTTCTTAAAAGGGGAATTATCTTATTTGCTGAGGAA11.1627906976744187No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA11.1627906976744187No Hit
GGCGAATCTGTTTGTGAATCTGATAGACATTCGTGGGTTCAAACTGCGCA11.1627906976744187No Hit
GGGAGAACAAGTTCCAAAGGTGCCGACTGGAAGAAGGGGGTAAAAGTTTG11.1627906976744187No Hit
CTCAGAACTGGGCCGTGGAGCCTATGGGGTGGTAGAGAAGGTGCGGCACG11.1627906976744187No Hit
TGATCATCAAACCTATACAAACAACTTACCAGAAAATACAGATGAGAACC11.1627906976744187No Hit
CCTTCTATACGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCT11.1627906976744187No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT11.1627906976744187No Hit
GAAGAATAAACATAAATTAGAACATAAAAAAATAATTATACAAAAATAAA11.1627906976744187No Hit
GCTGGAAGGCAACTTCCTGTCGGCGCATACAATACCGGTAAGCAATTACG11.1627906976744187No Hit
ACTCGCAATATGCAAACTAACATAAGAAAAAATAAAATTTAGTAATTTTA11.1627906976744187No Hit
TTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCACATAAC11.1627906976744187No Hit
ACGGCGGCGGCGGGCCTGCGGCGGCGGAAGAGGAGGTGCGGGACGCCGCT11.1627906976744187No Hit
CCAGACTGGCCTTGAATTCCTGGGTTCAAGTGACCCTCCTGCCTTGGCCT11.1627906976744187No Hit
TTTGAAATAATCTTGAAGATATTCAATACATAAAAAATTATAAATGAATA11.1627906976744187No Hit
TCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAAT11.1627906976744187No Hit
AGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATAC11.1627906976744187No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA11.1627906976744187No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG11.1627906976744187No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC11.1627906976744187No Hit
GTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGC11.1627906976744187No Hit
AAAACGCTAAAACAAATAACACAGTAAATGTTATAACCGATTCGGCCATT11.1627906976744187No Hit
CAGATATAGCATTGCGCGTTTGAATACTACAGCATGGAATAACAAAATTG11.1627906976744187No Hit
TCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTG11.1627906976744187No Hit
ATTTTCAGTCCTATGATTTGGATCATATGGAAAAAAAGGACCACAGTCAT11.1627906976744187No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG11.1627906976744187No Hit
TTCTTACCCTCAAGGACAAAGGCGTGCTGCAGGAGGAGGAGGACGTGCTG11.1627906976744187No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA11.1627906976744187No Hit
TGCTGATTAAACAATACTCTCTCGTTCTTTTTTGTTATCCTTTTACTGCT11.1627906976744187No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGTTTGGACAAGAGTTAGCCGCTCC11.1627906976744187No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA11.1627906976744187No Hit
ACTCGCATATTTTTTGCTCATGTGTAGTTTTTTTTTTTTTTTTTTGTAAA11.1627906976744187No Hit
GAGACTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTT11.1627906976744187No Hit
CTTTGAAAAAAAAAACTATAAGAAAACTTTCAATCAATAAAATAAGACAA11.1627906976744187No Hit
CTATAATAAAAGCAACAATTCAACAATAATCCCCAAACCACATAATCCAG11.1627906976744187No Hit
GCTCAATATCTAAAGCTCAAAATTAAGACTAATGAATGTTCAAATCATTC11.1627906976744187No Hit
CTCGACCAGCCGGAGAACGTCGAGAGCGTCGTCTGCTACGTGCAGCCCAA11.1627906976744187No Hit
GAAAATGAAGCGAAATTACAAGCAACGATTCGATCATAAAACAAAAAACA11.1627906976744187No Hit
CCTCGCTTCGTTAACCGCCCATTTGTACAATACGCAATGTTCCGGCGAGC11.1627906976744187No Hit
CGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCAATG11.1627906976744187No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG11.1627906976744187No Hit
TATGAAAATGTCTATAATGAGCATCATTTAGGAGATGTGCCTGCGGTAAG11.1627906976744187No Hit
CATGTACCCGCTCAACGACATATACCTCAACATCAACAACGTCGACATGG11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)