FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-076_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-076_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTTTTTCGTGAGC23.508771929824561No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT11.7543859649122806No Hit
AAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAG11.7543859649122806No Hit
TTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGA11.7543859649122806No Hit
GCAGGGTGTGAGCAGGGCTGAGATGGGCACCGCGGATCTGGGGCCGTCCT11.7543859649122806No Hit
TTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTT11.7543859649122806No Hit
GTCATGTGGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA11.7543859649122806No Hit
CTTTGCCTAAGACGTACATTCGTGCACACACACATGAGTGTGTGTACAGA11.7543859649122806No Hit
GTCATCGACGAAGATAACCTGTATGATCACTCCAATAATTTATTGCTCCC11.7543859649122806No Hit
TCAAGTCAATAAAATACAAACACGCACAACAAAAAAAAATTTAAAATGAA11.7543859649122806No Hit
CACGTAATGGATCCGTCCATTTTTAGTGTGTATCAATCGAGTTTCTGACC11.7543859649122806No Hit
CCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTAATTTTTA11.7543859649122806No Hit
TTTTGGCTTAGTTACGATTAATAGGAGTAGCTTGGGGGCATTTGTATTCA11.7543859649122806No Hit
TGTGTGTCTAACACAAGGAAGTTTAAGGCAACAACAGGTCTGTGATGTCC11.7543859649122806No Hit
GGAACATTTCCACAGAAATGTCATACATAATGCACGATAGCTATCAAAAA11.7543859649122806No Hit
GGAGTGTGTCAGTAATCCTATGAACCACAACCACCATCTTAAGAAAACAA11.7543859649122806No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT11.7543859649122806No Hit
CCTGAAAATGGGTCCGTTTATTTTGTGTCTTTATTTCCTCTTGAACCTCT11.7543859649122806No Hit
GCGATTAATGCCATTCTGAAGGAGGACATAAGGGCCTCCTTCCATGTGGA11.7543859649122806No Hit
TTTCAACACGGAGGACAAATTCAACCAGGGGTCCAGTGAAAATGTAAAGC11.7543859649122806No Hit
TTTTGGTCTTTATGAATATATTTATATGGACAGAATTAAGATAAACAAAA11.7543859649122806No Hit
GAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCT11.7543859649122806No Hit
TTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTAG11.7543859649122806No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA11.7543859649122806No Hit
GCGCATTTTGCTATTATGTGTTCTTTTAATTAAAATGATTCTTTTTAAGG11.7543859649122806No Hit
GGAAGTCCTTGTTACTTTGAGGAAATTAGAGTGTTCAAAGCAAACAGATA11.7543859649122806No Hit
GTTTCGCACGTGGCAGCCCTTTTGAACCCTTGGCAAAGATGCGCCCAGGG11.7543859649122806No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCGTGGGAATAGTCA11.7543859649122806No Hit
TTCTATGTTAAAGTGATCCTCCTACCTCAGCCTTTCAAAGTGTTGGGATT11.7543859649122806No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA11.7543859649122806No Hit
GGGAAGCAGTAAAAATCAAATAGTTGTGCACAAAAGAAATAGCCAAGTTC11.7543859649122806No Hit
GGTGTACCCACAGAGCATTTAGCAAAGGTAACCATTCGTGTAAGTTTACA11.7543859649122806No Hit
AACCAAACAGTCATCAACATCAATTACAGCTAACGGATCAACAAGCACCA11.7543859649122806No Hit
CCAAGCTGGTAATTTATTACCCCAAGGAGGTGGTGAAGAATTATACCTTA11.7543859649122806No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA11.7543859649122806No Hit
CCCACTCACACAAATCCACTCATCCCCATTCTCCTCAAGCCATTTAGCAC11.7543859649122806No Hit
AGATAATCTACCAAAAAAAATTACAAAATGCAAAATCACAATACATATCA11.7543859649122806No Hit
ATTGTAAGAAAATATGCATTTCATCCCCGTCGCGAAAGGGAAACAGGATG11.7543859649122806No Hit
TCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAAT11.7543859649122806No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA11.7543859649122806No Hit
GCAAGAGGAAGCAAAAGTTGCGCACCTTCCCATGCTTGGGGGTTGAACGC11.7543859649122806No Hit
TTTGAGTGTAATAGAGATGGTAACATACATACAAATGAACTCCTTCACAT11.7543859649122806No Hit
GCTCAAAGAGAACAGAAATCTCTTGTAGACTAAAAGGGGAAAAGCTTGTT11.7543859649122806No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT11.7543859649122806No Hit
CTGGTGTTCCGGTACCTCGTGCGGTGGTTGCTGTGACCGCGGCAGGGGGC11.7543859649122806No Hit
AAGGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGT11.7543859649122806No Hit
GGAGAAGATCTCATATTCGATGGTGTCAGTTTAGAAATTAAAGAAATATC11.7543859649122806No Hit
TTCATCCCCGTCTCGAAAGGGAAACAGGGTGATATTCCTGTGCCGATAGT11.7543859649122806No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC11.7543859649122806No Hit
AGACCCTATAATTGAAATGAAGTAAATTAAACCCTGAAATTAACATAATA11.7543859649122806No Hit
GCTTGTTTGATTTCTATTTTCAGAAAAAGTAGAAAACGTGAAAGCGTGGC11.7543859649122806No Hit
TCCCCAACTAAATCAAACATAAAAAGAGTATATATATCCAATGTTAATTA11.7543859649122806No Hit
TTAAGAATCATTTTTTGAGATTTCCAAGGAATTAGAGAACCTACGAAAAT11.7543859649122806No Hit
TCTTGATTCATTTCTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAG11.7543859649122806No Hit
AAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCC11.7543859649122806No Hit
CTGGTTTTATAACTCTTCTCAAAAGGGGAATTATCTAATTTGCTGAGGAA11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)