FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-063_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-063_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTTTAAGGCCTGCCCTAGCCAGGGCTCCCTCCTGCTTCCAGTACC11.694915254237288No Hit
GGAACATATGAGTAAACTGCTGAAAAAAGTTAAAGCGAAATGGATCCAGG11.694915254237288No Hit
CAACGATGAGGCTGCTGTCATTTGTGGTGTTGGCTCTATTTGCTGTCACT11.694915254237288No Hit
GGAGTTTGCCACTGTCCTCAGGAATCTTGAAGATGAACGGATACGGATGA11.694915254237288No Hit
GCATGACCCCTGTAACTGCTATCCGGAAGAGTCGCATGTGGCCCTGAGCC11.694915254237288No Hit
CTAAAAAAAAAATATCAAAACAAGGGTGGGGTGTTCCAAATCACTACCAA11.694915254237288No Hit
GAAAAAGAGGCCTTAGTACAACAGACGCTTACTACGCCACAGCCACCAGC11.694915254237288No Hit
CGCCAACGCTCTGCCGACTGAAAAATAACATTCCGGTCTTTAAATAAAGA11.694915254237288No Hit
CACCACTATTCACAATTACAAGGCACGACTGAACCCGTTTAAACCAAATT11.694915254237288No Hit
GTGGGGGTGGGGGAAAGAAAAGGAAGGAAGAAGTTCGCCAGCAGATAGCT11.694915254237288No Hit
GGGGGATTGCTCACCTCCACAGTGATCACACTCCATGTGGGAGGCCCAAG11.694915254237288No Hit
TTAAAATCCTACTTCCCTTGAAATCAAGATATTACTGCACACTCTATGAG11.694915254237288No Hit
AGAGACTCTGCTGAGTGGACCATGATGGAATTTGGACTTGGACACTGTCC11.694915254237288No Hit
TAAAGTTTGCTTGGTAGAAATAATTAATAATTTAGATATATATAAAAATA11.694915254237288No Hit
GTTCATCTAACGTAAAAGTATATTAATATGTTTCGCGCATGTGGAGATCC11.694915254237288No Hit
ATATATTTTTCTGATGTCTATGTTACCTTAGAGCGAAGGAAAATATGGAG11.694915254237288No Hit
TTTGTTGCTACTACGTGAGCCCTGCATTCACCTAGACCTGGGTCACAGCT11.694915254237288No Hit
AGAATAATCAAATAAAAAAATAAAATATTTAACTACTAAAAATGAAACCC11.694915254237288No Hit
AATCATAAAACATTAATTGAAAGATTATTTTTTAAAAATCAAACATAATC11.694915254237288No Hit
GTTACCTGTAGTCCCAGCTGCTCAGGATGCTGAGGAGATAGGATACCTTG11.694915254237288No Hit
TGTTAATCTAAAAGTGAGTCCTAAAAGAGGACGACCTGCAGCTACAGAAG11.694915254237288No Hit
CCTAAACTTCCAGAAAACGCACCGGAACTCTCACAAAGAACTGCCAGGGG11.694915254237288No Hit
CGAATATGAAAAACACAAATAATAAAGACACTTTGAGTACAAAAACACAA11.694915254237288No Hit
AGGGTATTCCTGGATCAATGGAGATCCGAGGCCAGACTCCCACCCTGTCA11.694915254237288No Hit
CATGTGAAGACATTGTGCATTATATCAATGTGCATTCCTGTAGTTCATTA11.694915254237288No Hit
CTGCCAGTTTCCAATTGACTGTGAAAGGGCTGTGGGTGTTTATGAAATAA11.694915254237288No Hit
CATTAATAAATAAATTCTTGAAATAAACATATCAAACCTTCAAAAAAACA11.694915254237288No Hit
CTAGAATATAATACAGAGGACAAAACAAAAACAGAAATTAGGATTTTGAT11.694915254237288No Hit
TGGAGTTAAAAACTATTGATGAGAAGGAAGAGCTGAGAAATATTCTGGTA11.694915254237288No Hit
ACTGCCCTGGCACCCAGCACAATGAAGATCAAGATCATTGCTCCTCCTGA11.694915254237288No Hit
CTTCCAGTGCATCGTGTACTTCTGGCAGGGCCGTGAAGCCTCCAATATGG11.694915254237288No Hit
CCTGTGAGATGGTGATGGACATCCTCCGGGAACGTGACCAAGGCGGCTTT11.694915254237288No Hit
CCCTCTGTTCCCTTATTGCTGCTTGTCACTGCCTGACATTCACGGCAGAG11.694915254237288No Hit
GTCCACTCCAGTGTGGCATCATGTGGCAGCTGCTCCTCCCAACTGCTCTG11.694915254237288No Hit
GGAACATATAAAAAATAACTTAAACTCAGAATTTAGAAAGACATACTATA11.694915254237288No Hit
ACGAGACGAGACGCGCCCCTCCCACGCGGGGACGGGCGCCCGCCTGCTCT11.694915254237288No Hit
GAAAATTTTCAACAAGATATGCTAGGGTCTTGCAGGATATGAGTTTGAAT11.694915254237288No Hit
ATTTAGTTTGGTAGGTCCAAAAATAAGTTTAAATATCAATAACACATAGC11.694915254237288No Hit
TTTTAAACCCACCAAAATTTGTCAAAAAAAAAAACTCTACGAAGTCCAGA11.694915254237288No Hit
AGGAAAAAATCAGACATATAATTATAAGTTACAATGGAACTCATTCAACA11.694915254237288No Hit
TGTTAAAGGAAAATTTAAATGGAGACTGGAAAAATCCTGAGCAAACAAAA11.694915254237288No Hit
CCGGCCGCCCGAGTGACCGGGAGCCGGGCCGCGGCCTTCCCTCGCCCGCT11.694915254237288No Hit
CATAATAAACGCATCACACATAACATCAAAGTACTTACTCACCAACAGCG11.694915254237288No Hit
AGACAGCCGCAAGTGTTGGACACAGATGCAGGATTTCTTCACAACTCTAC11.694915254237288No Hit
GGTGCCTTACGACTGCCCTCTTCACCCTCTGCATTACATCATGCCAGTAC11.694915254237288No Hit
CTCAGAGGCTGGGATCATGGTATATGTAACACTCCTTTTACTCCTCTCGG11.694915254237288No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT11.694915254237288No Hit
GCGAGGACAGCGCGGGCCCCCGGGCCTCTCCCCTGAGCCGGCCGCCTGGG11.694915254237288No Hit
GGACTGTTCGTGAACACCTCAGCCCCCCGCCAGCTCCGAACCTTTGTCCT11.694915254237288No Hit
CGATTCTCTTCCCCTGTTGATCTGGGTCATATCCAAGAAGGATGCTCCCC11.694915254237288No Hit
ATTTAATACCCACAGGAAGCCGGATATAATTAATTCTATTAGGCTGTGCA11.694915254237288No Hit
CTCCGAGGCAATAGTGGATCCGTTTTGGAGCGGCCTGGCAGAGTTGTGGC11.694915254237288No Hit
TTTGGACTTTGGAGTTTATGTAACCAAGGAGCCAGGCTGTGGACCTCGTT11.694915254237288No Hit
CCGCCCGCCTTGGCCTTCCAAAGTGCTGGAATTACAGGTGTGAACCACTG11.694915254237288No Hit
TGAGGAAGTAAGACAAAACCCTCCCCTCTACTCTCCTGGCCTAGTGAAAT11.694915254237288No Hit
GTTTGGTTAACTTTTATATACAAATGGATAAGAGACAATTATAGAAGAAG11.694915254237288No Hit
GTTTGTTAGTTTTTTTCTGAGACGAAGTCTCTCTCACAGCCCAGGCTGGA11.694915254237288No Hit
AGAGATATGAGATGAGTTAAAATTTGAAAACAGATAAAAAAGGCAGAATC11.694915254237288No Hit
TCCACATCCAAAATATACTACTATTCTCAATCCATCACTGGACTATCACA11.694915254237288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)