FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-062_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-062_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG601.4336917562724014No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG350.8363201911589008No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA330.7885304659498209No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA300.7168458781362007No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC270.6451612903225806No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC250.5973715651135006No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA230.5495818399044206No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC200.4778972520908005No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT180.43010752688172044No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG170.4062126642771804No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA170.4062126642771804No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT170.4062126642771804No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT170.4062126642771804No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT150.35842293906810035No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG150.35842293906810035No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA140.33452807646356036No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA140.33452807646356036No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT140.33452807646356036No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA140.33452807646356036No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA130.3106332138590203No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT130.3106332138590203No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT120.2867383512544803No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC120.2867383512544803No Hit
GTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCAC110.2628434886499403No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA110.2628434886499403No Hit
CTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATC110.2628434886499403No Hit
TGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACA110.2628434886499403No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA110.2628434886499403No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC100.23894862604540024No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA100.23894862604540024No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC100.23894862604540024No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG100.23894862604540024No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG100.23894862604540024No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA90.21505376344086022No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT90.21505376344086022No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA90.21505376344086022No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG90.21505376344086022No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT80.19115890083632017No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT80.19115890083632017No Hit
ATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTT70.16726403823178018No Hit
TGTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATT70.16726403823178018No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG70.16726403823178018No Hit
CCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACATATATACAA70.16726403823178018No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC70.16726403823178018No Hit
CGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCT70.16726403823178018No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG70.16726403823178018No Hit
GCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCT70.16726403823178018No Hit
GGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGA70.16726403823178018No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT70.16726403823178018No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT70.16726403823178018No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT60.14336917562724014No Hit
CCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAA60.14336917562724014No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG60.14336917562724014No Hit
GCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTC60.14336917562724014No Hit
GTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATT60.14336917562724014No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT60.14336917562724014No Hit
CGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGC60.14336917562724014No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT60.14336917562724014No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT60.14336917562724014No Hit
TTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACAT60.14336917562724014No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTTTAGCTTAATCCA60.14336917562724014No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC60.14336917562724014No Hit
CTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTAATT60.14336917562724014No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA60.14336917562724014No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT60.14336917562724014No Hit
TTTCAAAGAATCGATATTTTAAGCAACGCTTTTAGCTTAATCCACATAAC60.14336917562724014No Hit
TAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACT50.11947431302270012No Hit
CTCCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACC50.11947431302270012No Hit
GGGGACAACTGGTTTTATAACTCTTCTCAAAAGGGGAATTATCTTATTTG50.11947431302270012No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG50.11947431302270012No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG50.11947431302270012No Hit
TTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCCC50.11947431302270012No Hit
AGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAG50.11947431302270012No Hit
GCTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAATGATTAGAGGA50.11947431302270012No Hit
CTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTT50.11947431302270012No Hit
TTTTAAGCAACGCTTTTAGCTTAATCCACATAACTGATACTTCGTATCGA50.11947431302270012No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC50.11947431302270012No Hit
CTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTC50.11947431302270012No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA50.11947431302270012No Hit
TCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCC50.11947431302270012No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT50.11947431302270012No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT50.11947431302270012No Hit
CGGATGCATTCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAG50.11947431302270012No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC50.11947431302270012No Hit
TGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAA50.11947431302270012No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC50.11947431302270012No Hit
CCCGTCTCGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTG50.11947431302270012No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC50.11947431302270012No Hit
TGTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCT50.11947431302270012No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG50.11947431302270012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)