FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-061_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-061_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG241.079136690647482No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA110.49460431654676257No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA110.49460431654676257No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA80.3597122302158274No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC80.3597122302158274No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG70.3147482014388489No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC70.3147482014388489No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT70.3147482014388489No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC70.3147482014388489No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA60.2697841726618705No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA60.2697841726618705No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA60.2697841726618705No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG60.2697841726618705No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA60.2697841726618705No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT60.2697841726618705No Hit
CTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAA50.22482014388489208No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA50.22482014388489208No Hit
GTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATT50.22482014388489208No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT50.22482014388489208No Hit
CGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCT50.22482014388489208No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA50.22482014388489208No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA50.22482014388489208No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG50.22482014388489208No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC50.22482014388489208No Hit
GCGGCAAATACGATATATTCTTACGTGGGACTGAATTCGGTTGATTTGCT50.22482014388489208No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT50.22482014388489208No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA40.1798561151079137No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA40.1798561151079137No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG40.1798561151079137No Hit
ATTTTTTTGGGGTTTCGGCTCTGAATTCTTAATTTCCTGTTTTGTAGTAA40.1798561151079137No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG40.1798561151079137No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC40.1798561151079137No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG40.1798561151079137No Hit
GGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGA40.1798561151079137No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC40.1798561151079137No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG40.1798561151079137No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT40.1798561151079137No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT40.1798561151079137No Hit
TGAGCTGTGAGCTGGTAATGCAAATTATTAGTTTGCCGTTTCTGGACTAT30.13489208633093525No Hit
GTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTATACGCAC30.13489208633093525No Hit
GCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTTTGTA30.13489208633093525No Hit
TAAACAAGCACCCTCGCTTCAGTACCTTAACAAGAAATATCCGACATAGG30.13489208633093525No Hit
GATGAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTA30.13489208633093525No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG30.13489208633093525No Hit
GCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTTTGCT30.13489208633093525No Hit
AGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAG30.13489208633093525No Hit
TTTTGACTGAACTGGGTGTTCACTTTTTCCTTTTTGATTGAATACAATTT30.13489208633093525No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT30.13489208633093525No Hit
ATGGTAGCCTCTGGTCCTTCAACTACTTCTTCTACAACAAGCGGCTCAAG30.13489208633093525No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC30.13489208633093525No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT30.13489208633093525No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC30.13489208633093525No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA30.13489208633093525No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT30.13489208633093525No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACGCTTCTAGCTTAATCCA30.13489208633093525No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTAT30.13489208633093525No Hit
CTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCA30.13489208633093525No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG30.13489208633093525No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA30.13489208633093525No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT30.13489208633093525No Hit
TTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAA30.13489208633093525No Hit
GGCTGCCCAGTCAGTACAAGGAGGCCCACAAATATTTATTACAATCAGTA30.13489208633093525No Hit
TGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATC30.13489208633093525No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT30.13489208633093525No Hit
TGTCCTTAGATGAACTAGGCTGCACGCGTGCTACACTGATATGTATAACG30.13489208633093525No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTA30.13489208633093525No Hit
ATTGCGCGTTTGAATACTACAGCATGGAATAACAAAATTGAACAAGTCAG30.13489208633093525No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT30.13489208633093525No Hit
ATTCGGTTGATTTGCTTACTTCGAAGAAAATATTGGGATACGTAACAGTT30.13489208633093525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)