FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-053_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-053_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAATCGTTTAACCCAAAACAAAAACCAATATTCCACACATACTGAGTG14.0No Hit
AAAAAGTCAGCTAGCACCCCTGACTTCAAGTCCTCGAGTGTATGTTGGGC14.0No Hit
GAGTTTTGGGAAGTCCTGAAGGCGGATTTCCAAATGGTCTGCAGAGTAAG14.0No Hit
GATCCCAGGTACTAACGACCTCACCATACACGCATTCTTGATTAATTTCC14.0No Hit
GTGCAGGCTGTCCCACCTCCCTCCTCACCCCACTGGAATCGGTGCAGGCT14.0No Hit
TATGCCATCATTCATATATGAAGATCAAGTTCCACAGGCAAAACAAATAA14.0No Hit
CTGACAACAACAAATCTCACTATTAAAACAAAGCAGAAGCTGAACAAAAA14.0No Hit
ATGCAATAAACAGATGAAAAACAAAATGAAGAGAGCGAATCTGAAAGATA14.0No Hit
CCCAGAGGCTCTTTGAACAAATCAATGTGATCCTTTTTATAAATAGGGAA14.0No Hit
AGCGATACTACGACTCAAAAAAAAAAAAAAAAAAAAAAAACATCCGTCAA14.0No Hit
CGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCC14.0No Hit
TAAAATTCATCCAGGAAAATACTCATTTCTAAAACATTCGTTTCGTCAGG14.0No Hit
GTTAAATAAACTAAAACATCATCCAACTTAACGTCCAGTCTCCACAAACA14.0No Hit
CTTAATATGGTTATAATAATTTGAAATATATAATGAAAAAATTTAATAAG14.0No Hit
TATTAATTTCATGTTTTGAAGTAATAGTAATTCGATTTTATGAATTATCA14.0No Hit
AACTCATCTTTCCAAAGAAAAAATGCAAACAACCAGATCAAACCCAAGTA14.0No Hit
AAAGCAACCAACTACAAAACAAACACACAATAAAACAAAAGTATCCAACC14.0No Hit
AGTGAATTGATCCGGGAGTTGAAGAGGGCTGCAAGGTGGGAAGTGAAGTC14.0No Hit
CCAAACACAAGAACCAAGGAAATCCACACCCAAAAGCCTCCCAAACACAT14.0No Hit
AACTAATACATATAAAAAAACCACAAATATAAAAAATTACAAAAGAACAT14.0No Hit
GTAAGCCACAGCCTAATCTGGAACAAGGCCCTAACTTTCTTCAGTTCTAT14.0No Hit
TGCAGATAAAGGCACATTGACCTCCACAAATATAACACTCTCACTTATAT14.0No Hit
AACTCAGAACACAACCTATTGTGACAAAAAAAAGAGTGACTCACATATAA14.0No Hit
GCCCTTGCGCCTGCCTCTCCAGGATGTCTACAAAATTGGTGGTATTGGTA14.0No Hit
GAAAGATCTTCCATTCACAAAAAAAATGTCTTCCTATCACTGAGATGGAC14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)