FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-052_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-052_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTATAAAAAAATAAGAACAAAAAAACGAAGACAAAACAAACATCCGA13.7037037037037033No Hit
TCCGAAGCCGGAACAACCAACCCAGAAACACCCACGCAACAGCAATTAAA13.7037037037037033No Hit
ATTCAATGAAAATTACATAAATAGCAAACAATCCAATCAATATAATACGA13.7037037037037033No Hit
CGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTAGT13.7037037037037033No Hit
AGAACATCGCATAATCAGACTAAAAAAGCATTAAAAATAAATACAAAACA13.7037037037037033No Hit
CACTTTGCGAGGCTGAGTTGGGAAGATCACTTGAAGTCAGTAGTTTGAGA13.7037037037037033No Hit
GGACTTTGGTTTTTGCGTCTGTGATGTTGGAGGGCTGGGCCCCTTGAGCT13.7037037037037033No Hit
TTTAAAATGTCTGTTACATATCCAATACTGAACTAGCTGTGTCATTGCAG13.7037037037037033No Hit
GTAAAAACAACTAAAATAAAAAATAAACATAAAACAAATCAGAAAAAATG13.7037037037037033No Hit
CACAACACTTACCCGCACCTAAGCCAAACCTCAAAACTCAATATATCTGT13.7037037037037033No Hit
TATGCTACTAAAAATTCCTTTACAGATAAATTAAAAAAAAATTATGATTC13.7037037037037033No Hit
GGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA13.7037037037037033No Hit
CTGAAGTCGTACAAGAGCAACGCTCAATCAAAAAAGGCCAACTACTTCAG13.7037037037037033No Hit
AGAAAACTTACCTACTAAGTCACAGATGCAGCACGACTGTGCACGGCATA13.7037037037037033No Hit
CTTACAAAAAAATGACCCTATATAATATTAACTCTCAATTATATACAAAA13.7037037037037033No Hit
GTAAACATTTAAATAGAAAGACTACTAAAAGCAAATCCTTGATATGTAAA13.7037037037037033No Hit
CACAGCAGATCACCTCACAAAGTCCCAGGCAACACGCGGAGGACAGAGAA13.7037037037037033No Hit
AAATATTTTCTCGTTAAAAAGACAGGAACACAATTGCTCAACCCAGACCC13.7037037037037033No Hit
GAGAGACCCTGTATCTACAAAAAATTTAAAAATCAGCCAGGTGTGGTGGT13.7037037037037033No Hit
CTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCGGCGCGCTCGG13.7037037037037033No Hit
TTTTAAGGACTTACTATTGCTTCGGCGTGGAAGACCTTGATACTTTGAGT13.7037037037037033No Hit
AAAATAACACCAAACAACACAAAAAAACACACACAGTACATAAATTTGAA13.7037037037037033No Hit
TTTCGACACCGCCGTGTCCCGGCACTGCACCCTATATCCCAGAATCAACT13.7037037037037033No Hit
GAACTTTAAAACATACATATTCTTTGACCCAGAAATTCCACATAAAAGAA13.7037037037037033No Hit
GTGGTATATAATCAGCCCACATGGATCAGCTGGTCCCAGCTCTGAAGGTG13.7037037037037033No Hit
ATAGAATACCCTGATATGATATACCAAAGAACAAACTACTTCCCTTCTCT13.7037037037037033No Hit
CTCAAAAGCAAAAAGCCTCTGTGCTTAAACTCATACATATGCAACTGAAA13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)