FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-041_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-041_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGAATTTAATAAGAACTAACGGCGTCGCCAGATAGGATTCAGGTAG14.3478260869565215No Hit
GTTTTACTTCGCAGGAGCCTCATGGCCGTAGGCAGCCATGGCGCCCAGCC14.3478260869565215No Hit
AATAATTAAAAAACAATTCACATTCCTCAAAAAACCATCAAACCTAAATA14.3478260869565215No Hit
ATAACAGAACGCATTACCAAGGACAAACAAGGACCATTAGTCAGGCAGGA14.3478260869565215No Hit
GCCAGGCTGGTGCCACCAACAGTACAGTCCTCACCCTGATGACCTTGACC14.3478260869565215No Hit
GTAAAGAGTGTCCCCGAGAATCTGAACTTTAATCTGTGTTCTTAGTGATG14.3478260869565215No Hit
CACGCGGGGAAGGGCGCCCGCCTGCTCTCGGTGAGCGCACGGCCCGTGCT14.3478260869565215No Hit
CGGTGTCCCGACCCAGGCTAAGCTTGAGCATGGCTGAGCAGGAGCCCACA14.3478260869565215No Hit
AAAGCATTGAAAAATGGTACAAAATGGAAACAGTATCAAATCAGAGCTAG14.3478260869565215No Hit
CATTAACAAAATGTGAAATTGTGCAAATATTCTTATCTAAAAATATTCAA14.3478260869565215No Hit
CTTCGACACAGAAAAAGAGAAATATGAGATTACGGAGCAGCGCAAGATTG14.3478260869565215No Hit
GCTCCCGTGTCTTCTGTTACATTACCCAAGTAGCCTCAAATACTTAGACC14.3478260869565215No Hit
GTGATTTCCAAAGGCCCTTCCAGTTCCATTTGTCAGACATCTCATTGAGC14.3478260869565215No Hit
CTGTGTAAAGAGTTATTTAATCTCCTTCAACATAATCAAGTAACATCAAA14.3478260869565215No Hit
TGAATACAGACACAAAAAATTACCATAATCATAATAAAAAAATAATAAAA14.3478260869565215No Hit
AGGAGCTTGACGAGATCCGGCAGAAGTACCCGTCCCTGCGGCCGCCCCTC14.3478260869565215No Hit
CACCACTTCAAAAATGTGAAGGAGAGAACCAACTGGACCAACGAGCGAAC14.3478260869565215No Hit
CACAAATCTATAATCACACTACTTTGTCAGGCTGAGGCAGGTGTATCACT14.3478260869565215No Hit
AGCATTCAATAGAAATGTTTAAAAGAATGTAATCACAGAACTAAATAAGT14.3478260869565215No Hit
CAATCCGAGTAGCGGTATATTACAAGGAAAGGACACAGGTTACGGTGAGC14.3478260869565215No Hit
AAAAAAAAACATTAATCTAAAAAATAATTTTCATTTAAAACACTCATCTT14.3478260869565215No Hit
TATTCAAAAAACAAAACATCATCTTCCAAATAAGAATAATTCACCTAAAA14.3478260869565215No Hit
AGAAGGAATCAGCAAGTGGCCAGCAGTGTTACAATGTTGTACTTCCTGGA14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)