FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-034_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-034_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG130.563014291901256No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC120.5197055002165439No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG90.3897791251624079No Hit
GTGCAAGTGTGTATGATATGTTAAACATCTGGTACTTCGTTACCTTCTAT90.3897791251624079No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT80.34647033347769596No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG70.303161541792984No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG70.303161541792984No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA60.25985275010827197No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA60.25985275010827197No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC50.21654395842356No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC50.21654395842356No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA50.21654395842356No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT50.21654395842356No Hit
GTGTGTATGATATGTTAAACATCTGGTACTTCGTTACCTTCTATACGCAC50.21654395842356No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC40.17323516673884798No Hit
TGTGCAAGTGTGTATGATATGTTAAACATCTGGTACTTCGTTACCTTCTA40.17323516673884798No Hit
TTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTT40.17323516673884798No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTAT40.17323516673884798No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT40.17323516673884798No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC40.17323516673884798No Hit
TCTAGCTTAATCCACATAACTGATACTTCGTATCGACTTTGTGCGCATTT40.17323516673884798No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT40.17323516673884798No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC40.17323516673884798No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG40.17323516673884798No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT30.12992637505413598No Hit
GACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGG30.12992637505413598No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT30.12992637505413598No Hit
ATTTTTTTGGGGTTTCGGCTCTGAATTCTTAATTTCCTGTTTTGTAGTAA30.12992637505413598No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT30.12992637505413598No Hit
AAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTG30.12992637505413598No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA30.12992637505413598No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA30.12992637505413598No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG30.12992637505413598No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT30.12992637505413598No Hit
CTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCGCGTT30.12992637505413598No Hit
GTTAAACATCTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA30.12992637505413598No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT30.12992637505413598No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG30.12992637505413598No Hit
CTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTGGTAATGCAAATTAT30.12992637505413598No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT30.12992637505413598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)