FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-032_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-032_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTTTTCATGCTGCTTCAGAGAAAAAAACTAAATTAGCTACAATTAT16.25No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC16.25No Hit
AATGAATAAGCGTCGGAATGACCCGATATTCAACATACCTTCTGACACAC16.25No Hit
ACTCATTGCTACTATTTATCAGGTTTGTCAAAGATCAGAAGTATGTAGAA16.25No Hit
CCCGCCGGGTCCGCCCCCGGGGCCGCGGTTCCGCGCCGCGCCTCGCCTCG16.25No Hit
GGGGAGGATCATCCTAGAAGACTACAATGATAATGGTTTACTGCGCGTCC16.25No Hit
GGGTTCAAGAGAAAATAGGAGAGAACTGGGAGACAGAGAGCCATAGACAC16.25No Hit
GACCCTGTTGAGCTTGACGCTAGTCTGGCACGGGGAAGAGACATGTGAGG16.25No Hit
TGACAGAGCTTTTCTCTCCAATTGGAGTGTAAGCCCTACATGGCTAAGGT16.25No Hit
GGTTTTGTTTTTGTTTTTTTAACCAACTGCCCTATCTTGTGAAGCTGGGG16.25No Hit
ATGGATTATAAAATCCTAGAGAGATGTAGAGGAAAATCTTAATCATTTCT16.25No Hit
GTAGAATAAGAGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG16.25No Hit
CCCGCATCAGCCACCTTCCCTGTACTTGCCAAAGCCCTGGGAGAGGGAGC16.25No Hit
GGACAGCAGCACCAGCCCTGTGGGGCATGGAGTGGAAGCCCAGAAGGGCT16.25No Hit
CGGCGCAGGTCCCATCAGCTGGGGTTGACCCTCAGCCAGTGAGCAGCCAT16.25No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA16.25No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)