FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-031_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-031_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAATGCTAGTCGAAGGAGAAATGGACTCCATGATGTCGATTTGAACA18.333333333333332No Hit
ATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTT18.333333333333332No Hit
TTTTACTTCTAGAGTCGTGTTCTTTGAGATTGGAGCACTAATTCGTGTGA18.333333333333332No Hit
AAATTCTCAAGGGAGGAGAAAACCACGCGTGCAAAAAGAAGCCAAAGGAA18.333333333333332No Hit
TGACCATTACAACTCCAAAGGAAAGAGCTTTCTCTGATTTTTCTGGAAGT18.333333333333332No Hit
AAAAATTCCAAATAAATTAGGTAGACACTCCCCACTCCTTAGGCATGGGC18.333333333333332No Hit
AGTGGCCCCTACGGCGATCCGCACAGCGATCCGTATAGCGATCCGTATAG18.333333333333332No Hit
ATGGTAACATATATACAAATGAACTCCTTCACATAGGTCTTACACTCGGG18.333333333333332No Hit
CAGACGAAAGTCTGATGGAAGATCGAATTGATACTGACGTGCAAATCGTT18.333333333333332No Hit
GTTCAGTCAAAGGCTTGGAGTTTGGTAGACTCAGCTGATAATTCAGAAGC18.333333333333332No Hit
GGAGATTTTAGGTAAGAAGTTTCCTGTTACTGAGAACTCAAAGGGTTACA18.333333333333332No Hit
CAGAAGTGGGGTTCGCGCTGGTGGCATACGGATAATGCAACAATATGCAA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)