FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-028_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-028_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAAT11.9230769230769231No Hit
CATTGCGCGTTTGAATACTACAGCATGGAATAACAAAATTGAACAAGTCA11.9230769230769231No Hit
AACGGATTAAGAGAGAAAAAAAGTAAAGCGGGAAAATGATTCTTCGGATT11.9230769230769231No Hit
CGTCTCTGCAGTGGCACGCATAGCGGAATCACTTCCTGGGGTTGAGGCAT11.9230769230769231No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG11.9230769230769231No Hit
ATTTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTAC11.9230769230769231No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTA11.9230769230769231No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA11.9230769230769231No Hit
TGTCAACAATCCCCTGCATCTGGACTATGTGATGGCTGCTGCCAACCTGT11.9230769230769231No Hit
CCAGCGGAAAGGCGTCCAAGCCAAACCTCCCCGAGTCGAACATCGCAATC11.9230769230769231No Hit
AGGGAAGCGAGCCGTACCTCTCCAACGGACTACACAACGTGAACTCCCTC11.9230769230769231No Hit
GTGGTCGTTACGGAGAAGGGGGTATCCGATTTGGCACAACATTTTCTTGT11.9230769230769231No Hit
TTTTAAGGACTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTA11.9230769230769231No Hit
GGCCTCCACCTTCACAGTGGCTTCCACCCTGATGGCGACCTGCTTGTAGA11.9230769230769231No Hit
CTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTCT11.9230769230769231No Hit
CCACCCCCTGGATTTTCCTTCTCCCTCCATCCCTTGACGGTTCTGGCCTT11.9230769230769231No Hit
AAACATTTGGTACTTCGTTGCCTTCTATACGCACAATTAATGTAATATGT11.9230769230769231No Hit
ATAATTTTACCAGGTAGAGATAATGATTAGAGGAATCAGGGAAATCGTTT11.9230769230769231No Hit
TGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTC11.9230769230769231No Hit
CCGAGGCCCACCGGGAGGCAGAGGCCCAGGCCTGGGAGCAAGCCCGGCTG11.9230769230769231No Hit
GACCAGCCAGGTTGTTAACGTTACTGCTATATATATACGCATGTACTTGA11.9230769230769231No Hit
GGGAAAATGTCCCTCATGATCAACATTTCCGAGTGAAGAAATCGGGAGGG11.9230769230769231No Hit
GTGTAGCCTCCCCTGGCGCCTCATTGGTGGAAAATCCTAACAGAGGCCTC11.9230769230769231No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC11.9230769230769231No Hit
GCTTAACTGACGCTGAACGGAAGCCGAACTGACGTGAGCCGCCACCGAAC11.9230769230769231No Hit
CTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTAGAGATAA11.9230769230769231No Hit
GTGAAAGAAAATGCCTTACAAAAGGGGTTGAACGTGGCAAGTCATGTCTG11.9230769230769231No Hit
GTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGGGCTAAATA11.9230769230769231No Hit
GTTGGTTTGGAGGAGAGGTACCTCTATGAAAGAAAGCCTGGAAAACGCCA11.9230769230769231No Hit
TCTCCTTTCCTACCGCCTTGGGTTCTTCTCTTTTGAGCCCGAAAATCTAG11.9230769230769231No Hit
AATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAAT11.9230769230769231No Hit
CGTTGCCTTCTATACGCACAATTAATGTAATATGTTAATTATTGTAGAAC11.9230769230769231No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC11.9230769230769231No Hit
TTCGTATCGACTTTGTGCGCATTTTGCTATTATGTGTTCTTTTAATTAAA11.9230769230769231No Hit
GGAAAGTGGGCTCTAATGACTTGGTGTTGACCACCACGCCTGTAGGTGGG11.9230769230769231No Hit
ATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAG11.9230769230769231No Hit
TGTCGATGACGGTGCTGATGTCGATGAGGATGGCCATGATGACGGCAGGG11.9230769230769231No Hit
AGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTATTTTTTGGAATTTTAC11.9230769230769231No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGG11.9230769230769231No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA11.9230769230769231No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA11.9230769230769231No Hit
GCGACGATGGCGATGACGACGACGACGACGACGGTGTGGATGACGAAGCG11.9230769230769231No Hit
ATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATT11.9230769230769231No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC11.9230769230769231No Hit
ATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAA11.9230769230769231No Hit
ATTCAGAGTTGTTAAAAGGTCAGGGCATGTATCTTAACAAAGATGGACAG11.9230769230769231No Hit
TCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAA11.9230769230769231No Hit
GCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATA11.9230769230769231No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC11.9230769230769231No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC11.9230769230769231No Hit
CTATTCCTCATTTTAAAAAAAATAAAGCCGTTGGGATTATGAAGGCTGGC11.9230769230769231No Hit
CCTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAA11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)