FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-027_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-027_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTGAGGGTTTTTTACCCAAATCAGTCACTGGATTTTGCTGACTGATA14.0No Hit
CCTGGAAGTGCCCGTGACGCCACTCCAGCTCTGACCAGCAACAGCAACTG14.0No Hit
GTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCCGCCCGGCGGGCCGCCGG14.0No Hit
GAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCG14.0No Hit
CAAAAATTAACTCAGGATGGATTAAAGACTTAAATGTAAAACTTCAAACC14.0No Hit
ACTTACTCTGTGCTAAGTAATATTCTAAGTACTTGGCACACTTTAACTCA14.0No Hit
GAAAAAGCTTTCATATCTTACAGGAACCAGGGTAAAATAGAGATGTGAAG14.0No Hit
CGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCAGCGGGGCGCCCGGGCAA14.0No Hit
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT14.0No Hit
GGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGC14.0No Hit
GACCCACAGCAGGTTTGGGATTCAGCCGTGCTCCATCTGCCTGGGAGATG14.0No Hit
CAGGAAGTTCCAAACTTTCCCACATTTTCCTGTCTTCTGAGTCCTCCAAA14.0No Hit
AGACAACCTCGCCCTGCGGAAGGAGATCCAGTCCCTGCAGGCCGAGCTGG14.0No Hit
GGCAGGCCTGGGTTGGGTGAGGCCAGGAAGCCAGCAGGGAGGGGTGGGGA14.0No Hit
CACAAAAATAATGAATTGATGAGAAATACAATGAAGAGGCAATGTCCATC14.0No Hit
TGTGCAATGCCCTCAACAGCGTCTCGGAGAACCAGTCGCTGCTGCGGATG14.0No Hit
TAACATCGTTAACAGGTGATTTCAGAGACTGTCAGGGTCTGAGGTCAGGG14.0No Hit
CCGCTTCTTCCGCCGGAGCGTCATCTGCGACTCCAATGCCACTGCACTGG14.0No Hit
ATTGTCTTGGGCCGCACATAAAATATGCTAACACTGATGATAGCTGATGA14.0No Hit
AGGAGATTCTGCCCTCAGGCGTGTCTAGGAAGCCCTCCCTCCTGACCCTG14.0No Hit
TGAAAAAGAACCAGGGGTTACTAAAGAGCTGTTCACACAGAAGTAAGTGT14.0No Hit
AGAATATGGAAACTGAACAGCCAGAAGAAACCTTACCTAACACTGAAACC14.0No Hit
GTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGA14.0No Hit
CGATTAATCAATAAAGTCAAATTATTTTTTACGAGATTAAGCCTATAATA14.0No Hit
GGGAGTGGGGCCCTTTGGGCCACCAATGCCCCTGCACGTGAAGCAGACCT14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)