FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-020_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-020_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACACAACACCCATAGGTGTTGCCCCTACACTGAGGGAGGTATACACAA13.7037037037037033No Hit
ATGGACCCCTTTGACGCTTTAGTGATAGTTCAAAGAGGCCCCTTTTATAT13.7037037037037033No Hit
GAATCAGGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTA13.7037037037037033No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT13.7037037037037033No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATAAAATTTTACCA13.7037037037037033No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA13.7037037037037033No Hit
GATTAGAGGAATCAGGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCC13.7037037037037033No Hit
ATTTGTCTGGTTAATTCCGATAACGAACGAGATCTTAACCTGCTAATTAG13.7037037037037033No Hit
TTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGAC13.7037037037037033No Hit
CAAAATTATGAGGACAAAATGGGGCGTAAAGAATGCCCGAAGGCGAACTT13.7037037037037033No Hit
GCACTGGCAGCCCCCAGCTCAGCTCACCTATGAACCCCGTCAGCAGCAGC13.7037037037037033No Hit
CTTGACTGATGTGCGCTGATCCTAAAGCTTGTCGTGGAGACAGAAGGATG13.7037037037037033No Hit
CAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTG13.7037037037037033No Hit
AAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAAT13.7037037037037033No Hit
GGAAGTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCT13.7037037037037033No Hit
TGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATA13.7037037037037033No Hit
GGACCAGGACGCTTCCATCCCCTCTGGGGGCAGAGGCCTGTCCCCGGAGG13.7037037037037033No Hit
ACTGGAGCCTGTAACCCGTGCACAGAGGGTGTGGATTACACCAACGCTTC13.7037037037037033No Hit
AACGAGTTACTAAAATTACGTTTCAGCTTGCTGTTTCGTATTTTTCCTCC13.7037037037037033No Hit
TATGTGTGCCAGGCTGGCTGGCAGGAACTGGGGTGGCTATCCATATTGGA13.7037037037037033No Hit
ATAAAATAAAATGATACGTTTAAAGTACTTTCTTTGCTACGGATTGGAAG13.7037037037037033No Hit
GTTAATTATTGTAGAACTGGCTGGAAGGTAACTTCCTGTCTGCGCATACA13.7037037037037033No Hit
TTTCCATCCTTCAGGTCCCTCACTTTTTCTGGTTTCTCACTGTTAACCAA13.7037037037037033No Hit
TAACAATACAAGGCCAATCTGGCTTTGTAATTGGAATGATGGGAATTTAA13.7037037037037033No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT13.7037037037037033No Hit
CATTAGTAACGGCTAGTCAACAAGGACCATATCAAAAGGAAAATCACTCG13.7037037037037033No Hit
GTTACTTGGCTGGTTTCCTGGACAGAGAACATCCAAGGTTCCATTAAATA13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)