FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-013_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-013_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAGATACCACACAAGCAAATATTATTAAACACTAAATAAAATAAAAAA15.263157894736842No Hit
CCGAGTGCCGAGATAAATAAGAAAACCCCCGACACAAGGAGATGAACAAA15.263157894736842No Hit
AATGCATATAGTTAGAACTCTTTTCAAGCACTTAAATTTAGAAACCAAAC15.263157894736842No Hit
CAATAGTGATTACCTTGCCACATAACACCCAACCAAAATCACATTCAGTT15.263157894736842No Hit
CTAGGACTAGTCCAAGACCACCGCCACAACACAACCAATCAAGACTATAA15.263157894736842No Hit
TGTAACTGCAGACTTCATTAGCACACAGATTCACTTTAATTTCTTAATTT15.263157894736842No Hit
CTGGGCGCGGGCCTCCACGGGGGGCTGAAGTGGCTGAGGCCACTGCGCAA15.263157894736842No Hit
AATGTATAAAAACACCATTAAAGATCACAACACATAAAAGGACAGATAGC15.263157894736842No Hit
GTCACAGATGACCTCAAGGTCAGTGAAAAGTCAGAAGCAGCAGTGAAGCT15.263157894736842No Hit
CAACAAAACTGAACAAATATAAAAAAAAATTATAAAAAAGGTATGGAAGA15.263157894736842No Hit
TGACAAAAACAGAAACAATATTGTCAAAAGGTGAAAAATAGAACCAGTGG15.263157894736842No Hit
GATCAAGACACTCATCAAGAAGTACATCCAGAGGCAGGAGACCATCAGCC15.263157894736842No Hit
TTAAAAAGTGACAGCAAAAAGAATGATATTCCTTAATGCAAGTCACAATA15.263157894736842No Hit
AAACACACACCAAGAACTGCAGAAGTCAAATATTGCCATACAGCAACATC15.263157894736842No Hit
CCTAGTCCAATATGTCAAAAATTGGACAAAAGTTGCTCAATGATTAAGGA15.263157894736842No Hit
CTCAAGAAACATCACAAAAATTGCATCAATCCCCATCCCAAAAGAAAAAC15.263157894736842No Hit
ATTGCTTGAGCCTGGTACGTGTATGCTGCAGTAATCACATATTAAACCAC15.263157894736842No Hit
TCTCAGTTGCACAGACCAGGAAACTGAGGCCCACAGAGGCAGGTGTCCGG15.263157894736842No Hit
CTGGGCCACGTATAGTGATTTGAGAAAGTTTGATAAGAATCGATATGGAG15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)