FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_MHC-004_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-004_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAAA21.4925373134328357No Hit
CAATAAGAGGGAGGCCCACGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC10.7462686567164178No Hit
TACCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATGCAAGGC10.7462686567164178No Hit
GGCAGCTTTAACTCAGACCTGGTCACCCTGCCCCTCATCTCTAGCCTGCA10.7462686567164178No Hit
AAATATATTATGCTTTATCCTTCGGGAAGGCATTCTGATATTATTTGAAT10.7462686567164178No Hit
CCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCGGCGGG10.7462686567164178No Hit
GTCATGGAAGGAGACTGGGATAAGGCAGAAAATGACCTGAATGAACTAAA10.7462686567164178No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA10.7462686567164178No Hit
TTTAGTAACGGCGAGTGAACAAGGACCAGCTCAAACGGTTAATCCTTCGT10.7462686567164178No Hit
GGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATA10.7462686567164178No Hit
AAGAGAAGAAAAAAAGAGGGTCGAAAATAAAATTGCAGTCTTATTTGCTT10.7462686567164178No Hit
AAGAAAAGTCCTAAAACACTTTGTAAAATTAAATGTATAAAAAAGTAATA10.7462686567164178No Hit
TTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCA10.7462686567164178No Hit
GTAACACGTAATAAATTTATTTTATTTAGTGTGTATCAATCGAGTTTCTG10.7462686567164178No Hit
ATTTGTCTGGTTAATTCCGATAACGAACGAGATCTTAACCTGCTAATTTG10.7462686567164178No Hit
CAACTATTCAAATGAGAACTTTGAAGACTGAAGTGGAGAAGGGTTTCTTG10.7462686567164178No Hit
AGCTCAAACGGTTAATCCTTCGTTTGGTGCAAACCTTTCGACGGAATTGT10.7462686567164178No Hit
TATAAGGATAACTACGGAAAAGCTGTAGCTAATACTTGCTTTATTATCCT10.7462686567164178No Hit
GTGGGAGTAGAAACTGAAATATGTTTTTACGACAGTGAAATACCACTACT10.7462686567164178No Hit
GTGGATTCTAATTATTAGTTATCTCAGTTTATGTTTATCTGAACATGTTT10.7462686567164178No Hit
GGCCGCCCCCCCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCC10.7462686567164178No Hit
GGACGGGCGATGGCCTCCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGT10.7462686567164178No Hit
TAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACT10.7462686567164178No Hit
CTGTAGTCCCAGCTACTCAGGAGGCTGAGGTGGGAGGATCACCTGAGCCT10.7462686567164178No Hit
ATTAACTTAAGGAATTATAACAAAGAAGTAACACGTAATAAATTTATGTT10.7462686567164178No Hit
ACAATGTGATTTCTGCCCAGTGCTTTGAATGTCAACTTGATGAAATTCAA10.7462686567164178No Hit
CTGGATTTCGACAGAAAGACCAGACACAGAAGGCAGCCACCGGCCCCTTT10.7462686567164178No Hit
CTGGCCTGGCAGCCTTCCCCAGGTGAGCAGCAACAAGGCCACGTGCTGCT10.7462686567164178No Hit
CAAGCTGAGACTTCTCTTTGATTTACTCAGAAGGACTGGAAAATGTTGTC10.7462686567164178No Hit
TTTCTCCCCGCACTAATGTGGGGAAAACTGGCTTTATTTCTTCAATTATT10.7462686567164178No Hit
GACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGG10.7462686567164178No Hit
CCCGTATTACAGTGCCACGTTGGAAAACGCTACAGGAAGCATGACCTATC10.7462686567164178No Hit
AAAGGTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGA10.7462686567164178No Hit
CACCAGTGCCTGGCCAGCCTGCCCCCTTCCCTGGACAGGGCTGTGGAGAT10.7462686567164178No Hit
TAATTTGAGCAAAGTGAGCAGGAATGCTTCCCCATAGAGGGTGAAAGGCC10.7462686567164178No Hit
GAAATCTCTTGTAGACTAAAAGGGGAAAAGCTTGTTTGATTTCTATTTTC10.7462686567164178No Hit
GAAAGCGTGGCCTATCGATCCTTTATATTTGCAAAATGACGTAAATTACT10.7462686567164178No Hit
GAATCAGGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTA10.7462686567164178No Hit
CAAGAGTTAGCCGCTCCTAAGGGCTAGCTGAAAAGTGTTTAAAAGGGGGT10.7462686567164178No Hit
CACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAAC10.7462686567164178No Hit
CCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTC10.7462686567164178No Hit
GAGATTCCCACTGTCCCTACTTGCTATCTAGCGAAACCACAGCCAAGGGA10.7462686567164178No Hit
TAGGTGTTGGATGAAAGTGTTAAAAATAAAAGTCATCTTTCGAGGTGACT10.7462686567164178No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA10.7462686567164178No Hit
GAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAATTACCCACTC10.7462686567164178No Hit
GAGGAAGGGAGCGGCAGCAGTGAAGGATTTGACCCCCCTGCCACTGATAG10.7462686567164178No Hit
GTAGGTTGCTCTAAAAATATAAAGTACTGTATTCTAGCGTCCTTTAATAA10.7462686567164178No Hit
TTATATATCAAGATGGCTGATACAAATGGAAGAAATAGTATCGTAGGGCA10.7462686567164178No Hit
AGGGAATGAACAAAGTGGAGGTCGAAAGCGTCGTAGAACGAAAAATAAAA10.7462686567164178No Hit
GAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCTCGGTTGG10.7462686567164178No Hit
GGCCTAACATGGCTATGACGGGTAACGGGGAATTAGAGTTCGATTCCGGA10.7462686567164178No Hit
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGG10.7462686567164178No Hit
GCTTGATTGTAAAGCTTCTTAGAGGAACGATGTGTGTCTAACACAAGGAA10.7462686567164178No Hit
GTGGTGCTTTGCTTCTGTCCTGGTTGACCCTCTGCTGCTGGGCCATCAAA10.7462686567164178No Hit
CAAAGAAGTAACACGTAATAAATTTATTTTATTTAGTGTGTATCAATCGA10.7462686567164178No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG10.7462686567164178No Hit
GTTCGTTGAACTCAATTCAAAAAATTTCTTGAACGGGGTTTTCGGATTCA10.7462686567164178No Hit
GTTCATTTCAATCACGTTTAGGGGCGAAAGACTAATCGAAAAGCCTATTA10.7462686567164178No Hit
CAGCTTTTGATGTTAGGGTATTGGCCTAACATGGCTATGACGGGTAACGG10.7462686567164178No Hit
ACAAAATCAAAAATCAAAGCACCATTACCAGAAAACATATTTGGGAATCA10.7462686567164178No Hit
GCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATAACTATGATT10.7462686567164178No Hit
GGTGACCACCGTGAAGGTGCCTATGATGAAGCGTTTAGGCATGTTTAACA10.7462686567164178No Hit
GTTGAAGCGAAATACGTGAGTTTTCGTGGAACATCTCCCTAGTGCAGATC10.7462686567164178No Hit
TGAGCTATGTGGTTTCTTTCTTTCCATTTGGATTGCTCTTTCAGATCGGA10.7462686567164178No Hit
ATGGCTATGACGGGTAACGGGGAATTAGAGTTCGATTCCGGAGAGGGAGC10.7462686567164178No Hit
GAAGAACCTTTAGAGCTATAATCTAGTATGGCTTTGTGAAACTACATAAT10.7462686567164178No Hit
GGGTGAAAGGCCCATAGTTCGTTTTAATTTATTCATTGGAATTTTTCATG10.7462686567164178No Hit
GTGGAAACTCCCAATAGCAAATAATTTCTATTCTGCTTCTCTACTCTATA10.7462686567164178No Hit
TATTACTTGTATATTACATTTTAACCTGAAGGACCATTCTATAAAACATT10.7462686567164178No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA10.7462686567164178No Hit
ATTTTTTTTTTAGAATCTTTTTATGAGTAGAAAATCGTGGGGTTTGTGTT10.7462686567164178No Hit
GGGGAATCCGACTGTTTAATTAAAACATAGCATTGTGAAAAGCCACAACT10.7462686567164178No Hit
GTAAAAAGGGCGAAATAACTCAACTTGTATTTCACTCATATTAAATAAAA10.7462686567164178No Hit
GAAATTATATATGTACCTTTTTTTGGTACATAAATAATCCTATCACCTTT10.7462686567164178No Hit
AGAAGACCCTGTTGAGCTTGACACTAGTCTGGCACGGTGAAGAGAAATGA10.7462686567164178No Hit
CCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGGCCCTGCGGGCCGCCGGTG10.7462686567164178No Hit
TTCTTCCGTAGCTTCTGTTCTCGCTGCCTTAATAGGTGCTGGTGTAGGTT10.7462686567164178No Hit
TGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCA10.7462686567164178No Hit
TTTTAATATATTTTTTATATATACATATAGTATTGTGACAGTAATCCAAC10.7462686567164178No Hit
CGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGCTGG10.7462686567164178No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGATTAATATAATTTTACCA10.7462686567164178No Hit
GGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATT10.7462686567164178No Hit
CGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC10.7462686567164178No Hit
GAAAGGCGTAGGTAATCTTTATCAATATATATCGTGATGGGGATAGATTA10.7462686567164178No Hit
CTGAAGGGAACAAGCATCACAAGAGTACACCTCTACTCATTCACTGCAGG10.7462686567164178No Hit
AAATGTACCAAGTTGAAATGAATCAAGGGCAGTCCCAAGGTACAGTGGAC10.7462686567164178No Hit
AAGATATAATATTGGATAATACTAAAAACAATAACGAAAATTATAATTTA10.7462686567164178No Hit
GTATTGGCCTAACATGGCTATGACGGGTAACGGGGAGATCGGAAGAGCGT10.7462686567164178No Hit
ACCTGGGGCACCAAGACACCCTGAACCAGGGGGAATTCAAATAGCTGGTG10.7462686567164178No Hit
AGGACACCGTTTAAACCGCATTATCCATTAGTTATGAAATGTGCTGTCCG10.7462686567164178No Hit
AGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGC10.7462686567164178No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA10.7462686567164178No Hit
CATAAAAGGTGTTGGTTCATAATGACAGTAGGACGGTGGTCATGGAAGTC10.7462686567164178No Hit
CTTGCTTGATTGAAAAGCTTCTTAGAGGAACATTGTGTGTCTAACACAAG10.7462686567164178No Hit
CACTGATATATATAACGAGTTTTTAAAAATATGCTTATATTTGTATCTTT10.7462686567164178No Hit
TTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATGA10.7462686567164178No Hit
AAGAGAGGTAGTGACAAGAAATAACAATGCAAGGCCAATTTTTGGTTTTG10.7462686567164178No Hit
CGCTCCTAAGGGATAGCTGAAAAGTGTTTAAAAGGGGGTTCCTTCCCCGT10.7462686567164178No Hit
GCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC10.7462686567164178No Hit
CAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCC10.7462686567164178No Hit
GTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGT10.7462686567164178No Hit
GTTAATCCTTCGTTTGGTGCAAGCCTTTCGAAGGAATTGTCGTTGAATTA10.7462686567164178No Hit
AGAAGACCCTGTTGAGCTTTAATCTAGTCTGGCTTTGTGAAACGACTTAA10.7462686567164178No Hit
AGCCTATTAGCTGGTTATTTTCGAAAGATCTCTCAGGATCGCTGGAGTTG10.7462686567164178No Hit
GTTTTCTTGGTACGGGATAGTCAGAGTAACCCCAAAACTTTCGTACTGTC10.7462686567164178No Hit
TAAGGGATAGCTGAAAAGTGTTTAAAAGGGGGTTCCTTCCCCGTCTCAAA10.7462686567164178No Hit
AAACTTACAGTCAGTACAATGTTCAATATGATATGCCAAAAGAAGTATAC10.7462686567164178No Hit
GCAAGGGGTTCATATGGCTATATTAAAACAACTGCTGTAGAGATTGACTA10.7462686567164178No Hit
CTTAGATGAACTAGGCTGCACGCGTGCTACACTGATATATATAACGAGTT10.7462686567164178No Hit
CTTCTTTGGTGACCATGAGAAAAGGAAAGCTGGGGTAGTTCCATACAACT10.7462686567164178No Hit
GTCTGATGGAGGATCGAGTTGATACTGACGTGCAAATCGTTCATTTCAAT10.7462686567164178No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC10.7462686567164178No Hit
AAAATCGTGGGGTTTGTGTTGAAGCGAAATACGTGAGTTTTCGTGGAACA10.7462686567164178No Hit
CTCAAACTCCCAATAGGTAAAAAGGGCGAAATAACTCAATTTGTATTTCA10.7462686567164178No Hit
TTAATTTTTATTAAATCCCCACTTTGCATACAATACCGGTAAGCAATTAT10.7462686567164178No Hit
GGAAAGGAAGTTTTGGCAAGGTCTTCCTGGCAGAATTCAAGAAAACCAAT10.7462686567164178No Hit
ATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACT10.7462686567164178No Hit
GAGTAGTGAAAAGCAAATGGTTAAAGATAAGAGGAGGAGAAAAAATAATC10.7462686567164178No Hit
GTTCGTTTTAATTTATTCATTGGAATTTTTCATGCCGAGTCGTGTTCTTT10.7462686567164178No Hit
TGAGTTTCTTAGATGGTAACATATATATAAATGAACTCCTTTACATAGGC10.7462686567164178No Hit
AGCACGCGTGCAGCCTAGTTCATCTAAGGACATCACAGACCTGTTGTTGC10.7462686567164178No Hit
GTTTTCGGATTCAGTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTC10.7462686567164178No Hit
CAGACAGGAGGCCAGAGAGGAGTATTGCTCAATGCGTGCTATGTGCAACT10.7462686567164178No Hit
GTTAGAACACTATTATTATCGCTAGTTTTCCAAAAAGGAAACTGAGGCAT10.7462686567164178No Hit
TTCTAAAGAAGCAACAAAAGAAGCAAGTACTTCTAAAGAAGCAACAAAAG10.7462686567164178No Hit
GGAGTCTAGCAAATGTGCAAGTGTATATGATTCTTTAAACATTTCTCTTT10.7462686567164178No Hit
CAAAGCTGGAAGAGGAATAAATTACAAGTGGTCAAGGTTGCATCCTTTTG10.7462686567164178No Hit
CAAGTTTACGGCCTAAAGAATAATACAAAATCCTAATAACTGGAAATTAT10.7462686567164178No Hit
GCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATGAATGCCTTTAA10.7462686567164178No Hit
GTTACTCATCTTGGGCTCTTGTGCCAATAGCCCTTGTATGTATGTACTTA10.7462686567164178No Hit
CGTGAATATGATTTGTCTGGTTAATTCCGATAACGAACGAGATCTTAACC10.7462686567164178No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG10.7462686567164178No Hit
GAGAAGTTTTTACAAAGAACATGCTCACATCTTCAGGTGCCTCATTCACA10.7462686567164178No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)