FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_KHC-013_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_KHC-013_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTCCTCCTTGTTCTCTCCTTTTTCTCTTCTTCCTCCTCTGCTCCT12.5No Hit
GTTTGCCTGCCTTCTTGTGCGTTTCTCGGAGAAGTTCTTAGCCGGATGAA12.5No Hit
CCAAAAAGGCATAATAAAACTTTACTTCCTCTCTTTCTTCTTCCCACTCA12.5No Hit
CACTATTATAACTGCAAAAGACTGGAAAAAAATGTGTCTACCAATGGGAA12.5No Hit
GAACAGATGGTTCTATTGCCCAGCTTCACAAAGAGGAGTGTGGTCCTGGC12.5No Hit
TAGAGTTTAGCATTTCTATATCTTTCCACTGGCATGTTAAGCTAGCTTCT12.5No Hit
CCCGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATT12.5No Hit
ACAAATTGAACACAATATAAAATCAATTTCAAACCTAATTAATACCGAAA12.5No Hit
AGCGAATGGAAGTTGGGTGATTTTAAGTTATTGTTGCCAAAGAGATGTAA12.5No Hit
GATGAACATCAATGTAAAAATCCTCAATAAAATATTGGCCAACCGAATCC12.5No Hit
ATTTACTCTTTCTTCCCTACTCATAGTATGCGTTCCATTTTGAGGAATCA12.5No Hit
GTGACAACCAAGGAACCAACATTGGTACCTTACTATTATTATTACTATTA12.5No Hit
CTGTTAATAACGATGATCCATGTGTTTTACTCTAAAGTGCTAAATATGGG12.5No Hit
AAACTGATGCTCAAAGGAGAGTGGTTTTCCATAACTATGCAAAAATCAGC12.5No Hit
CATTGGTTTATGCAGGAATTAAATCAATTGTAAAGTCATCGTTGGGAATG12.5No Hit
GTACATAGGAATTGTGTAAAGTGTTAACAGCAGCTGTATTTGTTTAAATT12.5No Hit
GTTTAAGAATTTATAAGATGAGGAAGATGGCTTTTCCACTAGAGGGGATA12.5No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG12.5No Hit
ATCTTTACCACCTTGAGCTGTGAACCTGATATACCTAATCCACCAAGGAT12.5No Hit
AGTGCATCAGGTCAACTTGAATAAAAACACTATGACAGACAGGTTTGCCA12.5No Hit
TTTTAAGGTTATCAACTCAAACTTACCTGATGCTTAAATAAGCATCACCA12.5No Hit
GGAAGTTTATGAACAATAGAAATTTGTATCATTCAGTTCTGGAGGCTGGG12.5No Hit
GGCCATTGTTCAGCATGCTCCAGTCAGTGTGGAGGCCCATTGTCCAACCA12.5No Hit
CTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTC12.5No Hit
CCATGATGAAGGATTCGGCAGTGAGCATGAACTGTCTGAAAATGAGGAGG12.5No Hit
GGATTGTGCATATGGATTAGGCTTTATTTACTTGCTAATGCTAACCAAAT12.5No Hit
CTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGC12.5No Hit
GCTGGCCTGGAAATTAACAGGACTGTAGAGAACGTTGCAAGAGCAAAAGG12.5No Hit
AAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGG12.5No Hit
GTGGTCACCTTTGCTCCAGTCAACGTTACAACGGAAGTAAAATCTGTCGA12.5No Hit
CTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGTAGAAGAGGTTC12.5No Hit
ACTATGACATTTATAATGCGTGTGAGAAAATATGGGGTGAAGATCTAAGA12.5No Hit
GTTGTTAAGATAGACATTGAAGGACAGCAAAATAAGAGTAGAATTAGGTA12.5No Hit
TAGATGGTAATATTCGAAAGATAAATAATGATAGCTGGAGTAGACTAAAA12.5No Hit
AAGGTACAATAAATGGCTTACTGGATGAATTATTACAGACAAGGGTGCTG12.5No Hit
GACCTTCTTTAGTGTCTATGTTACTGCATGACCTTGTAGAAGTAATTTTT12.5No Hit
ATATAAGAGATCTAGGAAATGGCTAGAGTTGACAGACTGAGGAAGTGGAA12.5No Hit
CACAATTAATGTAATATGTTAATTATTTGTATAACTGGATGGAAGGCAAA12.5No Hit
CAAGCCTTTCTTTCATTCCCTCTCTGAAAAGTATTCCAACGTGATATTCC12.5No Hit
GAAAATAAGTGCGTGTTGTTCAAACCATCCAGTCTATGGTATTTTATGGC12.5No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)