FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_KHC-004_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_KHC-004_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47935
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA3690.7697924272452279No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG2090.43600709293835405No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1730.36090539271930744No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA1080.22530510065713988No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG960.20027120058412434No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA950.19818504224470637No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC820.17106498383227287No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC800.16689266715343695No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC750.15646187545634715No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA710.14811724209867527No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC640.13351413372274953No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA620.12934181704391362No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC570.11891102534682384No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG560.11682486700740587No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC560.11682486700740587No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT560.11682486700740587No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC540.11265255032856994No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT530.11056639198915197No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG530.11056639198915197No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA510.10639407531031606No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT510.10639407531031606No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA480.10013560029206217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)