FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n02_KHC-003_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_KHC-003_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA390.418140881312319No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG330.3538115149565777No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG230.24659590436367537No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA180.1929880990672242No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG130.13938029377077302No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC120.1286587327114828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)