FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MTH-023_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-023_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTTCACTGTTTTCAAGCTCAGAAAAACCCTCTACTGAAGCTTCAG15.263157894736842No Hit
CCCAATTATAAAACAGGCCACAGCAGACCCTCTAAGACTCCTTCCTTAAT15.263157894736842No Hit
GCGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGTCGCGG15.263157894736842No Hit
ATTTCTATGTAATTGGATCAACTTCTAAGAATTGCACTAGACTGATCCTA15.263157894736842No Hit
CCAGGATGATTTTCTCAAGCAAGTGCTGACAGAGCAGGAGTCAAGCGGGG15.263157894736842No Hit
CGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA15.263157894736842No Hit
CATGGCAGCTTTGCTCCTGCCTTCGCAGACAGGAATGCTGTCCTTCCAGC15.263157894736842No Hit
CCAGAATATATAAAGAACACTTACAACTCAGTAAAAAAAACACAAAAGCA15.263157894736842No Hit
AAACTGGTCCAAAACGATACCAAAGTACTGAAGTAGATTATAACTTTCAA15.263157894736842No Hit
GGATGGCAAATAAATATTTGAAAATATGCTCAACATCATTTGTTGGTAGG15.263157894736842No Hit
CACCTGACAAGCATGGACTCTACCAAAAATGCCTACCCAACTTTCTGAAG15.263157894736842No Hit
CTGCGAGCGGGTCGCTAACGGCAGCGCTACCATAACGGAGGCAGAGACAG15.263157894736842No Hit
GTTCAACGTTATAGTCAATTCTGTCAAGGACTGTACCCTGTTCTACAATC15.263157894736842No Hit
CTCACCCTGGAAAGGATGAGGGGGCGTCTCTCAGGCCAGCCCAGCCCCCA15.263157894736842No Hit
GGGCACGAACAGGAACCGCAGGCAGACCAGCAGGGGCCGCAACCGGCTGT15.263157894736842No Hit
CAGGCCTGGAAGCAGGGACTAATTTTTGAGGGAATTGCCAGGGATCTGGT15.263157894736842No Hit
GTCCGGAGAGCAGGGATGAGGGATAGGAGAGACTACCAGCCGACCAAGGT15.263157894736842No Hit
GCTAATTGATTATCATGTAAGCAAGCTATTTGAAGAAATTGATTACATAT15.263157894736842No Hit
CTGGAATAATGCTCGAAGCAACACATGATCTTATTTTGAAGACAAGTTTA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)