FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MTH-008_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-008_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTTTCATGTCTAATAAGATGATATGAACTCTTAAAGGCTTTTGGACA23.125No Hit
CACCGACGAGCTCCCTCAGGACCCACGCGCGGACAACTCCACGGCGACCG11.5625No Hit
CCCCCACCAAGTATATTCTTCATTAAGGACATAAATTCAAGAAGATAAAT11.5625No Hit
CACACCGTCCCGAACACAACGAGAACTAAAACACGGACCTCGAGCACCAG11.5625No Hit
GCTTTTCTCACCGAGGGTGGGTCACACTCCCCCCACCCGCCAGCCGCTCC11.5625No Hit
CTTTAATAGAAAGCCCAACAGATTTGGTATTAAATACATCTTTTCCATCA11.5625No Hit
CCTCTCGACTTCACAGCTATCTTCTGGCTCTCCACTGCTTCACTGTGGCC11.5625No Hit
CATTAATTTGAAAGGAGTTTCTCCCTTGTAGTTAGGATGTTAAGTTATTT11.5625No Hit
ATACGAAAATCCTCCCAACAAGGGGGATTAGGGACGTAGCAGCTCAGGAA11.5625No Hit
CAACTGTATAGTATCTTTCACCACAAGTAAATTTTAACATATTGTTCAAC11.5625No Hit
CTGAAAATCAAATGCATCAACCAAAGCAACAGCATCTGAGCGAATCAGAG11.5625No Hit
GCTTATACAAATAAATAATATAAATTATTAACAAAAATGTCAAGAAAAGA11.5625No Hit
GCTATGCTTCGCAGGCTGATGACAAACTCCTGGACACAAGCAATCCTCCC11.5625No Hit
AATTAACTTATAAGTGTTAATGGACAACTGTGCTTTGATTTTTGCCTTTA11.5625No Hit
CACTGCAGTATCAAAAAGTACAAGAGTATATTGTTCTCCACCAATCATAA11.5625No Hit
CACCATTTCTTTCTTTCTTTCTTTTTTTTTTTTTTTTTTTTTGAGACAGA11.5625No Hit
CCCGACGCTGCCGCGCGCCCGCTCTACCTCGCGAAGCACACAAGACAGGG11.5625No Hit
CCTGGCTATTCTGATGGACAAACTCAGTATCACCAGCAGAACTTTTCACT11.5625No Hit
CCCTCTTTTGTCTTTCCTGCTGCTTCTGCCACACCCTGTTTGGTTTTCTC11.5625No Hit
CTCCCTTTACCAAAACTCCCAAACCAACATCAAAACACAAGACTCCCCAT11.5625No Hit
GCTGCCGTGAACGTGAAGGGTATGAGCGAATACAGCGAATCAATTGCTGA11.5625No Hit
CTTCTCTTCCCCCGACCAGCATTTTGTCTTTCACACTCAAGGGGACTTTC11.5625No Hit
CCTAGCCCCTGGATTGGAGTGGAGGCAAGGAGGGTGGCGAGCTGTCTTCA11.5625No Hit
CCTTAATGCTCCATTCACGGCAGTGTAGGCTTTTTCTCGCCTGCTTCTTC11.5625No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA11.5625No Hit
CTGGGGGTGGTGGGAGGTATAAAAATTTATTATACATAAAGAATATTACC11.5625No Hit
CCAGTATGTACCACGGAGGACCTCCTCCTTCCCCACTGCCTGGACCTTGC11.5625No Hit
AACGCACCCCGAGTGTAAGACCTATGTGAATGAGTTCATTTGTATATATG11.5625No Hit
GTTCCTTGAACACAAGCTCTGTTATACCAGGACAGTCGAACTGATTACCA11.5625No Hit
CCAGTCTTCTTCTCTCTTGTACTCCTGTTCCAGGCTCTCGAGGACGCTGC11.5625No Hit
CCCTCTTTCCAGCTTCGACTGCCTTGTTCTTAGTGAGCACTGCCCGGGAA11.5625No Hit
CAGGCAGGCAGATTATTTTAATGCTGTTATACAGGGAATTGGTACTCTCG11.5625No Hit
CTACTAGTAAATCTATCTAACACTATATATCTATGCAAACCCTGAAATAT11.5625No Hit
AGAAGGGTCAAATCTTCCTTACAGTAGTACTACAAATAATCATAATCCAC11.5625No Hit
GTCCTGCAATACTTTCTTGATGGCGACCAGTTCTCCTGAATCACAAAGTT11.5625No Hit
CTTTCCCAGGATCCTGCTGCCGGCCGCCCCCAAACTGCTCCCCATAGGAC11.5625No Hit
GTGGGACTAGGTAGTTTCTCTCTAACAATGCTTCAGATCTCAATTTTTTT11.5625No Hit
GTCATAAATAAACAGGCAAATTATTAATTACAGGATTTGAGGCTTAGGAT11.5625No Hit
CTTCTGTTGAGTAAGTGTCCTTATGCTTTCTTTTCTCTTTGCTCTGATCT11.5625No Hit
GGCACTATCTCTTCAGAACTGCTGCTCTGGGTCTCAATGGTGGCTGGGGA11.5625No Hit
GGGGAGGGGAGTTAACCTTCCGGTTCTGGTGCAAATCTTTTTTCAGCCGG11.5625No Hit
CCCCGGGGTTCCTAAACGCGCACCTACTCCCCAACTACCTGAGCCTCAGC11.5625No Hit
GTCATATCAAAAGATCATCAAAAACCCAGCACAATACTAAAGCAACAAGG11.5625No Hit
CCTTTGTGTCCAGGAATTCCCCAGACAGTGAGGAGGGAGGAGAGGCCATT11.5625No Hit
GGTGGCCTGGCCAGCGAGGAGCAGAGTCACCAGGATGGAAAAGCCTGTGT11.5625No Hit
CATTAAAAATACTACTTAAACAAAAACATAAAAACTCTCACACACTAAAA11.5625No Hit
GTCCCGGGCTTACCAGATGACCAGCTAATGTTTTAAGTGAATGGATAAGT11.5625No Hit
CCAAAACATAAGAAACATTATTTAAGTAACATGAATCAATTTAATCCAAA11.5625No Hit
ACCAGAGTGAGTGCAGTGAGCTAGAAGTTGGCTGAGAGGAGGCAACACAT11.5625No Hit
GTCAGAGGAAAAGGCTAGAAATAATCTCCATGGCTGTCAACAGAGGAAAA11.5625No Hit
CTAGGAAATAACAGAATTTTAAGAGGGAAGACTTTCTAGACTCAGATACA11.5625No Hit
CCTCAGTCTAGATGAACAGATTAGCCTCAGTCTAGATGAACAGGCTGGCT11.5625No Hit
GTCAAAGAGGAAAAATGCAGAGAGAAACTGTGGTAAAATAGAAAATTATA11.5625No Hit
CTTAGAAGTTATCATTTCGCCTTCATTTTTGTCTAAAGCAGTCCAACACA11.5625No Hit
CTGGCATCTTGAATTTGGGACCTTTTAGTTTTGCGTCTGGACCTTCAATA11.5625No Hit
CTTGTTGCCTGCAGCTTTGAGAACACAGAAGTAAGAAGCAGTGTCTGCTG11.5625No Hit
GGCAATCACAACTTCAAGAAATCGAGACCATCCTGTCCAACAAGGTGAAA11.5625No Hit
AGGTTGAAGGTGGAAGTGGAGCCGAAGCTAGAGGTGGAACTGAGGCTGAA11.5625No Hit
CTGTGTGCTGCCCGGGGACCCATGAGACTTCCCATTAGTTCTGCAACCCT11.5625No Hit
TGCCGATGAAGCAACAAAGCTCTGGCCTTACAAGGCCTCAGAAATGGGGG11.5625No Hit
GTCATAATCTTAAAATAATAATACTTCTACAGAATAATATAACCAGGTTA11.5625No Hit
CTCTAACTGCAGACTTTGTTCTTGGTGAATTGCTATAGAACTGGGAATGA11.5625No Hit
GAAGAAGATGTACCAAGAAAAGCAGGTGACTGGGATTCAGAACATAAGGC11.5625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)