FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-091_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-091_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCTCCTTTTCCACCTGAGCTCTTCCTCCTACACATCACAACAGCCAT11.5873015873015872No Hit
CTCATTTCGAATGATTTTAAAAAATGCATCCAATGTTCCCTGGAAATTTC11.5873015873015872No Hit
CACATTATCCGATTGTAAAATGACAAATCCAATAAAGAAGCTCCAATACA11.5873015873015872No Hit
ATTGTTAACAGTTATCTTAACAATCTATTATTGGCAGTTTTCTTTTCATC11.5873015873015872No Hit
GTAAAAGCTGAAGGCAAGGTAGAACAAAGCGATGATACTGCAAATTCTGC11.5873015873015872No Hit
CCACTACCACGACTATCACTATCTACAAAACCTCAAATACATACAGAAGC11.5873015873015872No Hit
GAATAAAAAACATATTTAGCTTTATGTCACAACTAACCCACCAAATACTC11.5873015873015872No Hit
ACTCCATTTTACGACACATGGCAGGCAAGAAGACAACCAGCTCGATGGGA11.5873015873015872No Hit
CAAAAAAGGGGTTATTAAAATAGGACACCTTGAGACCATCGCTGAGTGGC11.5873015873015872No Hit
AACTCTATTACACACAAAATACTATAGGCAAACCAATATCAACCTGTTTC11.5873015873015872No Hit
GAAACATTAAAACATCCCGAAAAAAAAAAACCACACACATTTAAACAAAT11.5873015873015872No Hit
CTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGATGTGCAATCAAG11.5873015873015872No Hit
CACTCTGTCACCCAAGCTGGAGTGCAGTGGCGCAATGATAGCTCACTGCA11.5873015873015872No Hit
CAAATAGTAATAAAAAGATAAATTCAAAAAAAAAATTAATAGAAAAATCT11.5873015873015872No Hit
CACTGGAATACTGTGCAGCCATAAAAAAGGATGAGTTCATTTCCTTTGTA11.5873015873015872No Hit
CTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAAAGC11.5873015873015872No Hit
CCGCTGACTCGGGCACTGTGTTAATAGACCTACCGGTGCTGTAATTTTTT11.5873015873015872No Hit
TTACTTTTGCCTTGTAGCAGCACCCAATCTGGTGATAGAGGTAGCCATTG11.5873015873015872No Hit
TAACCATCACCATCAACATAAAAATCCAGCCCTGGTAGACGCTTAATATC11.5873015873015872No Hit
GGCACATCGTTCTGTCCTCTACCCTCTTTTTTTGTTTTTGGAGACAGGGT11.5873015873015872No Hit
CTGGAAATTAAAATTAAAAAAAATAAACGAAAAAAATTATATAACCAAAA11.5873015873015872No Hit
GTGTCATCGTCACCATGGGTCGGAGCACACGAAGTACAAGAGGACCATCC11.5873015873015872No Hit
ACTTAAAACAATAAGAAAACCTCAAATCAAAGTAAATTCATTCCAAACTA11.5873015873015872No Hit
AATAGCATTAGGTGAAAAGTATCACACGAATAATTGCTCCAATCTCAAAG11.5873015873015872No Hit
GCGAATATATATGCACCTTATCCAAGAGATAGGACCATCCCTAATCTCAA11.5873015873015872No Hit
GGAAAGGGGAGCTTAACGTCTGCTTCTCATCAGGTTGTACTCCTCAAGCC11.5873015873015872No Hit
ATTGGATTCAATACTATCTGTGGTTTCAGATATTCACTGAGGTTCTTGGA11.5873015873015872No Hit
TTACTATTTCAGGAGAACCAGTATAAAAAGCAAATTTTTCTAAAATAGCT11.5873015873015872No Hit
TTCAAACCTACTTCACAACCTATCAGACCACCTTACAATACAGGCCCAGA11.5873015873015872No Hit
CCCGTCCCAGCCATCCAACACTCTGTCCTTCCCTGCCCAAGGATGGACAC11.5873015873015872No Hit
TCATGATCCAGGTCGCAACGCGGAAGACCACGATGTCTTGGAACACAAAA11.5873015873015872No Hit
GGGTGAGCCGCTCGGGGGGAAGACGAGGATCGGCGGGCGGCGGGCGGGGC11.5873015873015872No Hit
CCACGATACCCGTCATAGCAATGTAAGGCCAATACACTAAAATCAAAAAA11.5873015873015872No Hit
TGATAGAAACAAACGATTGAAAAAAGTATACAACAAAAAACAGAATCAGG11.5873015873015872No Hit
AACCTTGTAGTATTCTGCTCTTCATTATATGAGATTCTAAAATTTCCCTG11.5873015873015872No Hit
GGGAAAAAATAAGGTTTCATGTGCTGGGAAATACTTAGCAGTAATAAGTA11.5873015873015872No Hit
GGCTAACAAACAATGTTTGCCAGGAGATTGATTACTCACTCTTCCTGTGA11.5873015873015872No Hit
CAAAGAAATATAATAAAATTAAACAGAGTAAAAAAAAAAAACATAAAGAA11.5873015873015872No Hit
TATTGTATCTGACCAACTCACTGATGCTTATTACTAAGAAAAACAGCTCA11.5873015873015872No Hit
CCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCAGGTCCCGGCTC11.5873015873015872No Hit
GACTAAAAAACATATTTATCTTTAACATCAATGTATAAAACTTTTAAGAC11.5873015873015872No Hit
CAACAACTCAAACACAAAACAAATAAAAAAATCCAAAATACATATAAAAC11.5873015873015872No Hit
TTCTAAACCTCTATACCTCACTTGAACAAAGTACTAATCAATATCCACAG11.5873015873015872No Hit
CTAAATAAAAAATAAATAAACTTACTTACACTTTGCAAACTTAACTCACC11.5873015873015872No Hit
GAAACTTTATTATTTAATATTTAAATATATTCAATAAAATTATATACACA11.5873015873015872No Hit
GCAACTTAATAATACAATTAATATTAATACCCGGCGAACAGAGTAATATA11.5873015873015872No Hit
GGCGCTACCATAACGGAGGCAGAGACAGAGGCGGCGGCCCGGGGGATCCG11.5873015873015872No Hit
CCTAATAAAAATAATAATACTAAACAAAAAAAATAAAAAAAAATAAATAA11.5873015873015872No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT11.5873015873015872No Hit
GCCATATCCGCCCTAAGTCAGCTGGGACTCCTTAAAGCCCAAAGGCTGGA11.5873015873015872No Hit
ATCCATTAAAGCATCTTCAAGTAACGGTGTTACGGCATAAATGTAGTCTT11.5873015873015872No Hit
ATCAGATTCAGTTTATTTTTTTCTTTTTTTTCTGGAGATAGAGTCTTGCT11.5873015873015872No Hit
ACAAGTACATCATGTCAAGAGGACAAACCATAACATAGAAATCGGCGAAG11.5873015873015872No Hit
CTGATGTCCATCTCCACTTCTGCTTGGGAAACCAGTCTAAGAAGTGGGTC11.5873015873015872No Hit
CCCTGAAGCGCGTCTTTCCTCGGCTCCTCTATCACCTCTACCTGGGGCTT11.5873015873015872No Hit
GAAGATGAAAATTGTATAGGGAAGGTAAATGGTTGATAAAGCTAGGGTGG11.5873015873015872No Hit
CTGCCCCAGACTCGGGCGCGCAGTTCCGACGGCCCAGGGCGGGGACGGCG11.5873015873015872No Hit
AGAACATATAATACCAAAAAAAATCTAAATAAAAAAACAAAAATATCATA11.5873015873015872No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG11.5873015873015872No Hit
TGTATTTTAGTGCATTCAATTTAATCTCCCAACATGAACATCAATCATCA11.5873015873015872No Hit
GATACTCATAATTCATAAAAACGAATTACTAAAACTAAAAAACAGGTAAT11.5873015873015872No Hit
CCACGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC11.5873015873015872No Hit
ACTCGGGTCCTACCAAATATGCTCTTAATCAATATAGCGTAATTGATTAC11.5873015873015872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)