FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-087_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-087_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA572.077259475218659No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC240.8746355685131195No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA210.7653061224489796No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT210.7653061224489796No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA210.7653061224489796No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC190.6924198250728864No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG190.6924198250728864No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC140.5102040816326531No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA140.5102040816326531No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC140.5102040816326531No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC130.4737609329446064No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT130.4737609329446064No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT120.43731778425655976No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA110.4008746355685131No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA110.4008746355685131No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA100.36443148688046645No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA100.36443148688046645No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT90.32798833819241985No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT90.32798833819241985No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA90.32798833819241985No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA90.32798833819241985No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA90.32798833819241985No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC90.32798833819241985No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT90.32798833819241985No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC90.32798833819241985No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT80.2915451895043732No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG80.2915451895043732No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT80.2915451895043732No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA80.2915451895043732No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT80.2915451895043732No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA70.25510204081632654No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT70.25510204081632654No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT70.25510204081632654No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC70.25510204081632654No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT70.25510204081632654No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT60.21865889212827988No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC60.21865889212827988No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT60.21865889212827988No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA60.21865889212827988No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC60.21865889212827988No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA60.21865889212827988No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC60.21865889212827988No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG60.21865889212827988No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG50.18221574344023322No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT50.18221574344023322No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC50.18221574344023322No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA50.18221574344023322No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA40.1457725947521866No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC40.1457725947521866No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG40.1457725947521866No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC40.1457725947521866No Hit
CACCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA40.1457725947521866No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT40.1457725947521866No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA40.1457725947521866No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA40.1457725947521866No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT40.1457725947521866No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC40.1457725947521866No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA40.1457725947521866No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA30.10932944606413994No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG30.10932944606413994No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA30.10932944606413994No Hit
GCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCT30.10932944606413994No Hit
CATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACAC30.10932944606413994No Hit
GCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTT30.10932944606413994No Hit
CGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTA30.10932944606413994No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA30.10932944606413994No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA30.10932944606413994No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA30.10932944606413994No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC30.10932944606413994No Hit
TTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTA30.10932944606413994No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA30.10932944606413994No Hit
CCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGAC30.10932944606413994No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAAA30.10932944606413994No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA30.10932944606413994No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT30.10932944606413994No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA30.10932944606413994No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT30.10932944606413994No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT30.10932944606413994No Hit
GCACCCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTA30.10932944606413994No Hit
TATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGACTT30.10932944606413994No Hit
GGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAA30.10932944606413994No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT30.10932944606413994No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT30.10932944606413994No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA30.10932944606413994No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA30.10932944606413994No Hit
TCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC30.10932944606413994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)