FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-086_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-086_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTACAGCGC18.333333333333332No Hit
CAAAATAAAAAGTTAATTCAACATCCCACAAAACCATAGAGTTTTATAAA18.333333333333332No Hit
GCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCT18.333333333333332No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC18.333333333333332No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG18.333333333333332No Hit
CTATCTATCATCTGTCCATTCATCCACCTACCTAACCAGACATACATAAA18.333333333333332No Hit
GTGGCTTTTAGGATGACAAGGGACTTCCTGTAACAACGCATCTCATATTT18.333333333333332No Hit
CTGTCTGTGACACTGTTAACGTATATAGTTATAATTGTTCTATTAAAAGT18.333333333333332No Hit
GAGACTTTGATCTTCTTGACGGTCTTGGTGGAGTTGTTGGTGACGTGGAC18.333333333333332No Hit
CTTCCATGACCACCGTCCTACTGTCATTATGAACCAACACCTTTTATGGG18.333333333333332No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC18.333333333333332No Hit
CCTCACGGTACTTGATTGCTATCGGTCTCCTACACATATTTAGCTTTATG18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)