FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-080_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-080_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGTTTAAAGTCTTAGAATACTAAACAAACTATGAAATTTTTATAAAC11.694915254237288No Hit
ATCTCATCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCG11.694915254237288No Hit
TCTAGTATGACTCTTTTACAGTAAAAGCAAAAGCATGGGTGTCTGTGTGT11.694915254237288No Hit
CGGGAACCAAAGGGCGGTGCAACCTGACGACTGGCCAGCCTGGGTCATGT11.694915254237288No Hit
CACATATCAACTAAAAAAACAAAATTATCACGATGAACTGCCAAATTCAT11.694915254237288No Hit
ACTACACTAAACAGCACTATTTACACACACAAATAAAAATACTTTGCCGA11.694915254237288No Hit
GTTAAGGGCAGCCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAGCCCA11.694915254237288No Hit
AAAAAAACCAGCTAAAAAAACATACATCTCTATAAAAAAAGTCTAATAAA11.694915254237288No Hit
GTAGATTTACAGAAATGTGTTTGAAAAATCCACGGTGATGTTCCAAAGTC11.694915254237288No Hit
CCGACATGAGGGAACAGCCTCTTTCTACTAACATCTGTCTTCGGTTTCAA11.694915254237288No Hit
CAATAAAATCCTAATTCAAACAAACATCTCCTCTCTTCAGAACTGCTACA11.694915254237288No Hit
CCACTGCCAAGTCTTACTTCTATGATTTTCTGATATTTGAAATTCACAAT11.694915254237288No Hit
GTGCTGACCTTCTGTATGAAGCGTGTTCACACAGTGCAGTGCTGACGGTC11.694915254237288No Hit
GTACTTTTCTTTGGAAAACAAGCCCTGTGGAGAGATCCTTCCATCAAGTT11.694915254237288No Hit
GCCAAAAATGTATGACTAATAACAATTGAAAGCAAGGCAGTGCTTCATAA11.694915254237288No Hit
GGTGACAGAGTGAGACTCTGTCTCAAAACAACAACAACAAACAAAAGAGA11.694915254237288No Hit
CAGTAACAACTTGAGAAAAATGGTTTATTTAAGAGTCTGTTAACAGATTG11.694915254237288No Hit
ATTTATGTACCAAAAAAAGGCACATATATAATTTCTCTTTTACATTAGCA11.694915254237288No Hit
CATAGCACAAATATCAATAATAACATAAACTACAAACAAATAATTTAAAT11.694915254237288No Hit
ACAAAATATCAAAAAAAATAACAAACAAAAACACAAAAATATAAAAAAAA11.694915254237288No Hit
CTCCAGTGCAGAAGTTAAAGGGCTGAAAAATTAACTGTCAGGAAAACAGT11.694915254237288No Hit
CACTTATAACAGGTTATAAATAAACTGGTTTTCAAGCATAGAGCCAGCCC11.694915254237288No Hit
GATCATCTTCTTCAACCAGTCCCTGAAGAAGGCAAATCGAGGCTTGTGAC11.694915254237288No Hit
CACAGACTCTGTAGGCTGGGCATTTTCAAAATCAAGGAAAAAATTTGGCC11.694915254237288No Hit
GGTAGTAGGCCAGAGGGCCGTCCGCTAATACACCTTGCAAAAGAAAAAGG11.694915254237288No Hit
CACAAAACTACAATAAAGAAACATAGAACATTCATAATCCGTATATTAAA11.694915254237288No Hit
CACAAATAAACAACAAATAAAACAAAAAAAAACAAAAAAACAAAAAACAA11.694915254237288No Hit
GTGGCTGAGGGCACAGGTTTTAGAAGGCTGTAGTTGAATATGGCTCCTAA11.694915254237288No Hit
AACACACCCACATTTATAGCAGGAAGATGGGGAGTGGGCTGCAAAACCTT11.694915254237288No Hit
GCGAATATTTAGGCACCTAATCCAATCGTTAGGAGCATCCCTCATCGCCA11.694915254237288No Hit
GGAGATGTGAGGCCGCAGGTGGAGATGTGGAGCTCCCCACAGGTGCGTCC11.694915254237288No Hit
CATCAATAGGTTAACTACCATGTACCAGCTCCCCATTATGATCCTCCCGG11.694915254237288No Hit
CAACAATAACAATCAAATCCATATAAGCACACAACAGTAACATAAATGTC11.694915254237288No Hit
GTGAATTTTAAAAGGAGGAAGAGGAGGCAGCATTCTCCTTCAAACAGGGC11.694915254237288No Hit
GTCTGATTATCCTGATATTGGATTTTCTTGTTTTATGTTACACCTTGAGT11.694915254237288No Hit
CTCAAATCTCTAACAGTGGAAGGCTTAAACAAGAATGGATGCTGCTAGAA11.694915254237288No Hit
GATGGAACTGAGTTGGTTTTAAGAGTTAGAAATGACGGTGGAAGAAAAAA11.694915254237288No Hit
CCCAGAACAGAAACTGCAAGATATCTCATTGGAAAATGCATCCTAGTTAC11.694915254237288No Hit
AACTATATCAAGCAAGAAAGATACATTACAGAAGGTAGGTTAGTCTGAGT11.694915254237288No Hit
CTGGGCTCCAGCGATTCTCCCACCTCAGCTTCCTGAGTAGCTAGGATTAC11.694915254237288No Hit
GAAAAGAAGAGAGATTCTGAAAAATAGTCAGAAAAGAATGGTAAATGGAA11.694915254237288No Hit
ATTCAAAGTCAGCTTCACAGCTTGTAGGGTAGGGATGTCATTTTGAGTCT11.694915254237288No Hit
AGAAGGGGGACCGGTGAGTGTCAGGGAAGGGATCTGGCTCAGGACAGGGC11.694915254237288No Hit
TATCATTAGTTCCCATTTTACAGATGGGAAAATGGATGCTCAGTGTGATA11.694915254237288No Hit
GACACAAGGGTGGGCTGTCTCTCCACCTCCTCACATTATGCTAACAGGGA11.694915254237288No Hit
GGGCGCACGTGCCTGTCCCGAGTCGTGCTGGAGTGTGGTGAACACTGCGA11.694915254237288No Hit
AATCGATACTCGCTTAAAAGTAGTTCATCAAAAATAAAAAAGACATAGCA11.694915254237288No Hit
GAACATCAAAATTAAAAAAATCAAAGAAAGTCCTTAATTAAAACGTAACA11.694915254237288No Hit
AAGAATTACACATCGATAAACATAGTAGAACAAAAGAAAAAAAAAATAAC11.694915254237288No Hit
AAAAAAAAAAAAATACATCAATAAGATAATTTGCTCATTATTCACACACT11.694915254237288No Hit
CAAAAAGTGGGCTAAGCACATAAATAGAAAATTCTCAAAAAAATAAACAA11.694915254237288No Hit
GCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCA11.694915254237288No Hit
CTTTCACCAACAGCATCTGCAGGCCTCCCGGGGACACCAGGCTTCCTTTT11.694915254237288No Hit
AAACACAGTAAATGCTTTAACTGTTTGCTTTGAACACTCTAATTTACTCA11.694915254237288No Hit
CCTCAATCCACTTGATCCCAACTCATCTCTCATTTATTTCGGCTTCTTTT11.694915254237288No Hit
GTGAGAGTGGTGTTGCTGCTGCTGGTCTGCCCCAAGGAAACACCCTGCAT11.694915254237288No Hit
CATTTGAAGAGCAGGACACATGAGTTCAGCGAAGGAATAACAGTTGACCC11.694915254237288No Hit
GCTGCCTGGTCTATCACAAAGCATTCTTACAAGACTAGTTGAGTTACTGT11.694915254237288No Hit
ACTGATTGACCTTCTTCCGTGTGAGTGTCTTCTTGTCACCATCTGAGAGT11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)