FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-078_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-078_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT22.3255813953488373No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC22.3255813953488373No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA22.3255813953488373No Hit
TGGATACCTGATGCGGTTGTTGGTACGCTCATAAAAGTAGTAAGAGTATA11.1627906976744187No Hit
CAATACATTTTACAAGTAAACTTCTTACCTAAACAAATAATACACAACAG11.1627906976744187No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC11.1627906976744187No Hit
CACATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA11.1627906976744187No Hit
ACGTGATGTGCGTTTGTACGTTTGTATGCACGTATGTATACGCCTGTATC11.1627906976744187No Hit
CCTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCT11.1627906976744187No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT11.1627906976744187No Hit
CTATAATTTCGTTACCATCCGAGTCATGAATTGCGGATTTTATTTTTATT11.1627906976744187No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC11.1627906976744187No Hit
GGTGAGAGTCGAGGTAGAAAGCCTGGAAGTTCTGAAACGTGAGGGGATGG11.1627906976744187No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG11.1627906976744187No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC11.1627906976744187No Hit
CAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTA11.1627906976744187No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA11.1627906976744187No Hit
CCCCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACT11.1627906976744187No Hit
CACTGGGCTCCACTTCCAGGGTGATGGTCTTGCCGGTCAGGGTCTTCACG11.1627906976744187No Hit
CTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT11.1627906976744187No Hit
GGCGCGGATAAAGTTCGGCAGGCTGTTGATCCGCTGGCAGAACCCGTTGG11.1627906976744187No Hit
CTCGTTATACATATCAGTGTAGCACGCGTGCAGCCTAGTTCATCTAAGGA11.1627906976744187No Hit
CTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAGCAACA11.1627906976744187No Hit
CTTTCGATCAAGAATAATTGAAGAAACAAAGGCACATGCAAACCCACTGC11.1627906976744187No Hit
CTGGGGCTGACTTGGAGTGTCTTCTGTCTGGCATTCTTTGTTTTGGGTAT11.1627906976744187No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA11.1627906976744187No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC11.1627906976744187No Hit
CCATGAGCTCCATGCAGATCCACACGTCTCCCTCTCTGAATAGTGCCCCG11.1627906976744187No Hit
CCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATAT11.1627906976744187No Hit
AATCATAATAAAAACACTCAAAATCAAATTAATACTACAAACAAAGTAAA11.1627906976744187No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG11.1627906976744187No Hit
TGCTGAAAAGGCTGCTGAAAAGGCTGCTGAAAAGGCTGCTGAAAAGGCTG11.1627906976744187No Hit
CAAAACATAACAATATAATACTGCATCAAACATTGAACTTAACAATAAAA11.1627906976744187No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA11.1627906976744187No Hit
GTCTTATTAATAAACACAAAAAAAGCGCTGAACAAACAATGAAAAAAAAA11.1627906976744187No Hit
GTTTCCCTTTCGAGACGGGGATGAAATGCATATTTCTTACAAAACATTCC11.1627906976744187No Hit
CTTGTCTAATCAAATCTCTGAAGTCCTCTTTTTCTTTATACGTTTTAGGT11.1627906976744187No Hit
TATGAATGCATCCGTAGAGGAAGCACGTACAATTTCTCTTTTACATCAGC11.1627906976744187No Hit
CACGAGGCAAAAAAGTCGTCTACAGTTTTTGCAATTCTTTTTTGTTGATA11.1627906976744187No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT11.1627906976744187No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA11.1627906976744187No Hit
GGGGCAATGGATGCTTTGAGGAGAGATAAAAAGTTAGGAGGGCAATGCGA11.1627906976744187No Hit
CCGAACAAAACAAACAATGCACACGTAACCAGTAGACCCTTGAAAATTCT11.1627906976744187No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC11.1627906976744187No Hit
CTCGAGACGACGTCAAACTCGAGGTTAATCATCTGCTCCAGCACTTCTAT11.1627906976744187No Hit
CGGCTGAAGAGATGAAAAAGGCCTATGTGTGAAGGGCCCAGGTTAGTTAA11.1627906976744187No Hit
GCCATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGT11.1627906976744187No Hit
TGCCAGGTTCTGGGCTAGGCTTAACATAAAGCTCACTTTATCCTTAGTAA11.1627906976744187No Hit
TATTAATCTTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGACTA11.1627906976744187No Hit
CCAAGACGTAGAAGTATACCCCCCCAAAAAAAATGGGAAGTACCCAAATG11.1627906976744187No Hit
ATACCTATCAGTGTAGCACGCGTGCAGCCTAGTTCATCTAAGGACATCAC11.1627906976744187No Hit
ATCAACCATATAAAAAAAAATAAGTCAAATCAAACTATCATGAAACATAA11.1627906976744187No Hit
CCACATTCCTTTCTCTTTGATGCTGTTTCCATAGTTTCCAGGCTGAGAAG11.1627906976744187No Hit
ATAAATTGTGAACACCGATGATACACTTTTAGCATGCTCTTATTCACGAA11.1627906976744187No Hit
GGGGAGTACATAATCGCCTCGTTACGTGGCGGGGGAGGGGGTGCTGCTCA11.1627906976744187No Hit
CCCAACCTACTCCAATAAATCAAAAATAACACCAAATAATAAAAAATAAC11.1627906976744187No Hit
GCCACTCGTGGGTTCAGAACCTTCAGCCTGTTCCTTTGCTTCAGGGAGCT11.1627906976744187No Hit
CTCCCTTGTATTCTACTTGGACCTTGGGCCTGCCAGCATCATTCACCACC11.1627906976744187No Hit
GCCGCCTCGGCAACGTTTTGCCATGGCTCCTCTGGTCAAACAAAGTTTCG11.1627906976744187No Hit
TCCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAAC11.1627906976744187No Hit
GTTGGAAATGGTTGTCTTGTGAGAGTGACAGAGTGGGTGAAGGGAGACCA11.1627906976744187No Hit
GGGGGAGTGGCATGGGGATTGGGGACCCCTGAACTACACAATTTGTGTTG11.1627906976744187No Hit
CAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAAAAATTAA11.1627906976744187No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA11.1627906976744187No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTGTTGTTGTT11.1627906976744187No Hit
GTCAGGTCGTCCACGCTCTCGTCCTCGGCATAGGGCAGGTAGTCAGGCTT11.1627906976744187No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC11.1627906976744187No Hit
CCACATTACACGACATAGCAATATAAGTCCAATACACAAACATAAAAAGC11.1627906976744187No Hit
GTTTGCTTTGAACACTCTAATTTACTCAAAGTAACAAGGACTTCCAAGCC11.1627906976744187No Hit
CACCAAACTCAAAGAAAACGACACTACAATAAAAATACAAAAAAATACAA11.1627906976744187No Hit
CCTCGCAGTTAATAGAATACATAGATTTCTTGAGGACCACCCACAGGCAC11.1627906976744187No Hit
GGTTGTGGCTCTTGTCAGAGACCTGTTTCTTGGCATGAAAGCACATAAAC11.1627906976744187No Hit
CATTAAATTCATTTACCAACATTTTTAAAAGAAAATTAATAACGCGTACG11.1627906976744187No Hit
GGGCGCGAAAAACTTGCAGGGGGGCCCTGCGCGCTTTGGAAAAGGTCGAA11.1627906976744187No Hit
CCAAACTTCTCCACAGCTTTCTCCTCAGAAAGGCCACAAGCACCATATTC11.1627906976744187No Hit
GATCATCACAACAAAAACACAAAAAAATAAGACCAATATCCAAATTAGAA11.1627906976744187No Hit
CGCCTGCAGGCGATGGCACCTCAGCTCCCAGTGGCCGCTGCCCACGCCGG11.1627906976744187No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA11.1627906976744187No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA11.1627906976744187No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT11.1627906976744187No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATCAGAAAAAAGAA11.1627906976744187No Hit
GTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAAT11.1627906976744187No Hit
CTGGATATGTCTCCATAGAGTCGGGAGAAAAGTGAAACACTACGAGAAGT11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)