FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-076_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-076_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA35.263157894736842No Hit
TGTATGTACTACTATCTACTGAGATAAAAAAAAAAAAAAAAAAGGCACTC11.7543859649122806No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT11.7543859649122806No Hit
GCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTT11.7543859649122806No Hit
CTTCATACACGCACGTATGTATTATGTATTATGTAAGTATGCAGCGCTGC11.7543859649122806No Hit
TCCCTCTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGT11.7543859649122806No Hit
ATTATGAATGCATCCGTAGAGGAAGCACGTATAATTTCTCTTTTACATCA11.7543859649122806No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGTTAAATT11.7543859649122806No Hit
GCCCAATGACAGAGACATGAAAGCTAGAATTCTCAATAAATCAGATAAGT11.7543859649122806No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG11.7543859649122806No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT11.7543859649122806No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA11.7543859649122806No Hit
TGAATAATATCAAGTAAAGTTTATATAATTTACGAAAACAACTATAAAAT11.7543859649122806No Hit
GTCCAATTTTTCCATCTCGGCATACAGCAAATTCTTCTGACTTTGCATAG11.7543859649122806No Hit
ACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT11.7543859649122806No Hit
CAAGAATTTATTCATCGAACCTCTTCTCCAACGAAAATGATTCCAAGGTT11.7543859649122806No Hit
GGCCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAG11.7543859649122806No Hit
GACAGATAAGCAGACAAATGGACAGATAAGCAGACAAATGGACAGATAAG11.7543859649122806No Hit
CCAATTTCGACTATGCAGGAGGAGTCCATTCCGCGTTACCACATTGTGTG11.7543859649122806No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT11.7543859649122806No Hit
GTCAATTAGATCAAATGTATAAATTAAAACATCTCAAAAAAAAACAAAAA11.7543859649122806No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG11.7543859649122806No Hit
GCCTAGCTCACAGCTCAAATAGTCCAGAAACGGCAAACTAATAATTTGCA11.7543859649122806No Hit
GCTGTCCGAACCGAGCTCCAAATTCGAAACGATGTTATTGTTGTTGTCCC11.7543859649122806No Hit
ACCGATGTGAAGTTCCTCCTTATGTGTTCCAAAAACGGGTTGTAATTTAT11.7543859649122806No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG11.7543859649122806No Hit
GAACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAA11.7543859649122806No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA11.7543859649122806No Hit
AAAAAATTAAAGATCAAATAAAAAGCAACCCTACTGAAAGACCTAGGCGA11.7543859649122806No Hit
GACACAATTTGACAATATTTCAACAGAAGAGGAAGAAATCACATAAAGCT11.7543859649122806No Hit
GTCGCTTCTTTGTTATAAATCCTTAAGTTAATAACTTTTTTCTTAAAAGG11.7543859649122806No Hit
TTTGCAAATATAAAGGATCGATAGGCCACGCTTTCACGATTTCTACTTTT11.7543859649122806No Hit
CGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATAAATGCAATT11.7543859649122806No Hit
ACCGGTCAAAGCATAATTCGTTTCTGGGTGGATGATAACTTCAGATCCGA11.7543859649122806No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA11.7543859649122806No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA11.7543859649122806No Hit
GCACCATTTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT11.7543859649122806No Hit
CCTCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA11.7543859649122806No Hit
CCCCTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT11.7543859649122806No Hit
ATAATCTTATTCTGAAACCCCAGGTGAGCACAGACCTTTTCTGATACATG11.7543859649122806No Hit
CACCCTAATAAATATAATCTAAAAATAATAAAAAGAAACATTGATATTTA11.7543859649122806No Hit
CTAGGAAGACAAGATGGGGAAGTGAGAGAATGGGGCAATCAATTTTGTTT11.7543859649122806No Hit
CCCAACTGTACTGTACCACACTTGGAACATACGGCTCGCAAAAACCGCCT11.7543859649122806No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT11.7543859649122806No Hit
GCTGCGCCTAACTGCCTACTGCCGCACTCACGTAAAAGAAGAAGCTGTCG11.7543859649122806No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC11.7543859649122806No Hit
CAAAAAATAACTAAAACACATACATAAAAAAGCAATTAAAAAAAACTAAA11.7543859649122806No Hit
ACAAAAAAATTCCATCCATCAATAGAAACCCAAACCATACCCAAGAAGGC11.7543859649122806No Hit
GTCAGTTTCGTTGTGGAGGCGGTCGTATTCGTAGAGCATTCTTACGGCTC11.7543859649122806No Hit
CATAGCAGCAATGGAAGCGAGGGTAACAGTGCACATGAGGTTGGTCATAC11.7543859649122806No Hit
CAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC11.7543859649122806No Hit
ATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTC11.7543859649122806No Hit
GCCTGATCTTTTGCTGTGCGTGATCTCAGCCTATCTTTTATCGTATTGGT11.7543859649122806No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGAAAAATTCCAAAAAATAGAA11.7543859649122806No Hit
GGTTTATTACGTATGTCTGGTTCTCAATTCCAACAGTTTAATGAAGATCT11.7543859649122806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)