FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-063_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-063_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATTTTGGTGTTTCCATGAAGTCTTCCTGCTGTGGATTCTCCACACCA11.694915254237288No Hit
GATTTTAGCAGTGACAAAAGTGACAAAAGTTATAAGTGACAAAAACTAAA11.694915254237288No Hit
CCTGTCACTAAAAAAAATACAAAAATAACACTACCAATTTCCTGAATCAA11.694915254237288No Hit
CAGATGACTGCCCACTCACTGAAGCGACTGGCTTAGTCTTATTGCTCAGG11.694915254237288No Hit
CCCTGGTTGTCCTCACTCTCAAAGGGAACCTTGAGGATGAAGCAGAACTC11.694915254237288No Hit
ACTGAATCGACATGACTAGGCGAAGACAGTCTTAAGAGACCCACTGGCTA11.694915254237288No Hit
GGCTCCACAAACTCAAACATCTTTCTCTCTTGGACTTCCTGCACCTTGAA11.694915254237288No Hit
CCACAAAACAGAAAATAAATATCAAATCTTCTCGAAACCAACACACTGGA11.694915254237288No Hit
TTCCTCATCTGTAATATGTGAATAGCAATAGAAACCACCTCATAGGGATA11.694915254237288No Hit
ATCCCCCTCCCCCAAAAGTCCCTGAAAAAACTACAACTTAAGGAAATGCA11.694915254237288No Hit
CCTGCCCACTGTTAGCCAGGTCATCCACAGACAGAGTAGGGTCCAGACGC11.694915254237288No Hit
CTGTTTGACTGATAATAGAGCCTCGAGCTTGAGCGTCCTGCTGTCCAGAA11.694915254237288No Hit
GGCACAGATTCAAAAAGATCAAGAACAGAGTTGGGAGCAAAAGATAAATC11.694915254237288No Hit
GTTCATTTAAGTGAAATGCTTCGATAAAAACCTCGGTGTCACTTGGTTAA11.694915254237288No Hit
GGGAACTCTGGTGGAATGACAGTTAATTCATCAACTAAAGGGATGATTGG11.694915254237288No Hit
GCAATTACTCCAATGGACATCTCTTCCACAGACCTCAACGTGAGAACTGC11.694915254237288No Hit
GCTATTTTCTCTAGAAGATGATTTGGTTTGGCCTTTTAGCCTGGTGCATT11.694915254237288No Hit
CTCTGATTGTAGTAGCCGCGCAGCGTCCGCAGATTCACTCGGAAAAACTG11.694915254237288No Hit
CCCATCTTGCGCAAGTTGGTCACGTGGTCACCCAATTCTTTGATGGCTTT11.694915254237288No Hit
CTCAGACTAAAAAAAAAAAAAAAAAAAGATCAAAATACAAAAAATAAAAA11.694915254237288No Hit
GGCTGTTTTAGAGGGAAGTTTTCCGTTCACATTGATTTCCTCTCTCAGGC11.694915254237288No Hit
GCCGGAAATGAGGCTCGGATGCCGGCGAGGAGGGCAGCTGCTCGGGACCC11.694915254237288No Hit
CCTAACTATCATCAGAACAGCAAGGAGGAAACTGCAACCAAGATCCAGAC11.694915254237288No Hit
GGCAGCAGGACATACAACACGCCCAGACACACAGCATAGCAGGGCCTCGA11.694915254237288No Hit
CTAAAAAAAAAAAAAAAAAATTAATCATTTGTGTTTGCATGCACAACATG11.694915254237288No Hit
CCGACTGTGAGGCGTGCGCCACTAACGTTCCGCTCAGAGGAGTGGGAGGC11.694915254237288No Hit
CAAAAATCTAGCCCCGTCTACAGGACAACATCAAACCGAAAAGACTGAAC11.694915254237288No Hit
CAGGTATAGGGGGGAGGGACACTTTGGAAGACAGTGACATCTTTTCACAG11.694915254237288No Hit
GACCTAAGTAAAAGTAGATGTTTACATTCCTTTCACTTTTCCAAATAAAT11.694915254237288No Hit
ACAAAATAAAAAAATAATAATACAAATTAATCAAAAATAATAAAAAATAA11.694915254237288No Hit
GCCTGGGGAAGGGAGGGGGCCCTGCGGTACGAGGAAACACCTGCGCGCGG11.694915254237288No Hit
CACATTAATCCCTCCTCCATATACGAATTCAGTAAAATACAATCACAATA11.694915254237288No Hit
CCGGCACCGAGTCGAGGCGGATGCGGCGGACGCGGCTCCCACTGCCGCCG11.694915254237288No Hit
CTGTGAAATGCGAGCAAGGTAGGACTTTGTTAGACCTACTTCTCCGTGGG11.694915254237288No Hit
ACCAATTCCACATCTCCACGGCTGGCGCCCCTCCCGTTCCCAGGGGGCAG11.694915254237288No Hit
TTTGCCATTCTGTAAATATGTGACCTTATGCAGAGCAGTGTTCTTCCAGC11.694915254237288No Hit
CCGAGGTGAAGTTGAAATAACCAGCCTTGAGAGGGCGCAGGACCACAGTG11.694915254237288No Hit
GCTCAGTACACGTAAAGTTGAGACAGAGATGGAGACATCCAGCCCACTTC11.694915254237288No Hit
GCCAATCTCATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGT11.694915254237288No Hit
GCCCTCTTCATCCTTCTTAGGCTGCTTTTTAGGTTGTTTTTCCTCTTGCC11.694915254237288No Hit
CCTGTCTGGGGGCCCCATTATATGAGCAATCTTCTCGGGCCCTGTCCCGT11.694915254237288No Hit
CTTCAACTTGGGCTTCACATAAAAGATCTTATATACACCTAACAAAATAA11.694915254237288No Hit
CTGAACCAAAGACTCGGCTTTCTGGGTGCACAAGTGGCCCTTTATTCGAA11.694915254237288No Hit
GAAGGGACTACGAGACAACAATGGAACAATTGTGGAAAAGAATGTGGACT11.694915254237288No Hit
CCAAAGGCCTTTGTAGAAGTGTAACTATGTCGGTATGTATAAACAATTGT11.694915254237288No Hit
ATAAAATATATAGAACTATATACAATTTATTTTCATTAAAACGTTTTTTT11.694915254237288No Hit
CCGAATCACACACCTGGACACATTTAATCCTCCAAACTACACAAGGGGGT11.694915254237288No Hit
CCCCAACAGCGAAACCCTCCACAGACACATAATGACACGTGCTGAATCCA11.694915254237288No Hit
CTTACTATTCAAAGTTTACTGACCTCCCCAGCCAGGCAGGCCAACCCTTC11.694915254237288No Hit
ATTTATACTAAACCAACCAACAATATTCCATCTAATAAGTAACACTGGTC11.694915254237288No Hit
GCAACCACAGGGACACACATACCTCCAAACTCCTCTACATTCAGGAGGCC11.694915254237288No Hit
AGCCAAAGTAAGTAACAAAAAGAATTTCAATGGGAATTCCAAACTCTTGG11.694915254237288No Hit
GCACCGGTCACGACTCGGCACGGGAGCGGGCGGAGAGCCGACTCGCGGCG11.694915254237288No Hit
TGTACTGTTCTCTCTACAGATCAGATCAGTATTTTACAGTCAAGGAGTCA11.694915254237288No Hit
GTTCATGACTGTCGCATTCAGCATCTGGTCCCCCAGCGCCAGGTAGACCT11.694915254237288No Hit
CACAACCTCAACCACACATGTCTGAGAAAACAATTACTAATATCATAGCT11.694915254237288No Hit
ACAAATTCCCAACTTCAGAAAACAGGAAACTATAAGCATGTTAGGCTAGC11.694915254237288No Hit
GTGAAGTTTTGCTTGTGTTTGTAGTTTAAGCAAAGTTAAAGCTATTTCTA11.694915254237288No Hit
CCAAAACAATCTAAAAAAGAATCATCACACAATCGAAAAAATTGCACTTA11.694915254237288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)