FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-062_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-062_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA892.126642771804062No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT461.099163679808841No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA380.908004778972521No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT370.8841099163679809No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG360.8602150537634409No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC320.7646356033452807No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA310.7407407407407408No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC310.7407407407407408No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC300.7168458781362007No Hit
CCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCC260.6212664277180406No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA260.6212664277180406No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA240.5734767025089605No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA230.5495818399044206No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA210.5017921146953405No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT210.5017921146953405No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA210.5017921146953405No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA190.4540023894862605No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC170.4062126642771804No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCT170.4062126642771804No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT170.4062126642771804No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC160.38231780167264034No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT160.38231780167264034No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC150.35842293906810035No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA150.35842293906810035No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA150.35842293906810035No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT140.33452807646356036No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA140.33452807646356036No Hit
CTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCCA140.33452807646356036No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTG130.3106332138590203No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT130.3106332138590203No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT130.3106332138590203No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC130.3106332138590203No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA120.2867383512544803No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA120.2867383512544803No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT120.2867383512544803No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA110.2628434886499403No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT110.2628434886499403No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG110.2628434886499403No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG100.23894862604540024No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA100.23894862604540024No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC100.23894862604540024No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC100.23894862604540024No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA90.21505376344086022No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT90.21505376344086022No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC90.21505376344086022No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT80.19115890083632017No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT80.19115890083632017No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA80.19115890083632017No Hit
TCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTC80.19115890083632017No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG80.19115890083632017No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC80.19115890083632017No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG70.16726403823178018No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA70.16726403823178018No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT70.16726403823178018No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC70.16726403823178018No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG70.16726403823178018No Hit
TCTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGC70.16726403823178018No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT70.16726403823178018No Hit
ATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCTT60.14336917562724014No Hit
CTTACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACA60.14336917562724014No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG60.14336917562724014No Hit
CGGCAAACTAATAATTTGCACTACCAGCTCACAGCTCACGAACAGCAGCT60.14336917562724014No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGTGGAAGCACGTAT60.14336917562724014No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT60.14336917562724014No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT60.14336917562724014No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC60.14336917562724014No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT60.14336917562724014No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA60.14336917562724014No Hit
GCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGT60.14336917562724014No Hit
CGCACCCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTT60.14336917562724014No Hit
TATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGACTT60.14336917562724014No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC60.14336917562724014No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAA60.14336917562724014No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT60.14336917562724014No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG60.14336917562724014No Hit
ATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTC60.14336917562724014No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG60.14336917562724014No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC60.14336917562724014No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA50.11947431302270012No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA50.11947431302270012No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT50.11947431302270012No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA50.11947431302270012No Hit
GTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGAACGA50.11947431302270012No Hit
GCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTT50.11947431302270012No Hit
ACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGTGGAAGCACGTATA50.11947431302270012No Hit
CCAGAAACGGCAAACTAATAATTTGCACTACCAGCTCACAGCTCACGAAC50.11947431302270012No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA50.11947431302270012No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA50.11947431302270012No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC50.11947431302270012No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC50.11947431302270012No Hit
GCGCAATGCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTAA50.11947431302270012No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA50.11947431302270012No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC50.11947431302270012No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA50.11947431302270012No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT50.11947431302270012No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC50.11947431302270012No Hit
TCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCT50.11947431302270012No Hit
GCACCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTT50.11947431302270012No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT50.11947431302270012No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA50.11947431302270012No Hit
CCTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTT50.11947431302270012No Hit
TCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC50.11947431302270012No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA50.11947431302270012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)