FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-061_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-061_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA231.0341726618705036No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC170.7643884892086331No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC160.7194244604316548No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC150.6744604316546763No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT130.5845323741007195No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT130.5845323741007195No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA120.539568345323741No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT110.49460431654676257No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG100.44964028776978415No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA90.40467625899280574No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT80.3597122302158274No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC80.3597122302158274No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT80.3597122302158274No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA80.3597122302158274No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA70.3147482014388489No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT70.3147482014388489No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA70.3147482014388489No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA60.2697841726618705No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC60.2697841726618705No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC60.2697841726618705No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA60.2697841726618705No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG60.2697841726618705No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT60.2697841726618705No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA50.22482014388489208No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG50.22482014388489208No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT50.22482014388489208No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT50.22482014388489208No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG50.22482014388489208No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT50.22482014388489208No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT50.22482014388489208No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT50.22482014388489208No Hit
GCAGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACTAGGCATTCC50.22482014388489208No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA50.22482014388489208No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA40.1798561151079137No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA40.1798561151079137No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA40.1798561151079137No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT40.1798561151079137No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT40.1798561151079137No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC40.1798561151079137No Hit
GCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTAACAAGGAC40.1798561151079137No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA40.1798561151079137No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC40.1798561151079137No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA40.1798561151079137No Hit
CCAAAAATGGCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATAC40.1798561151079137No Hit
GCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGT40.1798561151079137No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA40.1798561151079137No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG30.13489208633093525No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT30.13489208633093525No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT30.13489208633093525No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC30.13489208633093525No Hit
AGCAGGCTGTACAAGACATGTTTCAGTATGGCTCTTTTCTGTTCTTATTC30.13489208633093525No Hit
GTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGTCAG30.13489208633093525No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG30.13489208633093525No Hit
CCCCTGCTTCTGCTTTCTTCCTCCACCTCCTCCTCCCCCAGCTCCATGTC30.13489208633093525No Hit
CTAAGAAGCTTTACAATCAAGCAAATACATTCTTACGCGAAAAAGAAACA30.13489208633093525No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA30.13489208633093525No Hit
TCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGC30.13489208633093525No Hit
CCTGGAGACAGCAATTGCCCATTCCAAATCCCATGGCATCCATGTAAATA30.13489208633093525No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC30.13489208633093525No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT30.13489208633093525No Hit
TATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAA30.13489208633093525No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA30.13489208633093525No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA30.13489208633093525No Hit
CCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGAC30.13489208633093525No Hit
CTAACATCAAAAGCTGATAGGTCAGAAACTCGATTGATACACACTAAAAA30.13489208633093525No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA30.13489208633093525No Hit
TCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTA30.13489208633093525No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA30.13489208633093525No Hit
TTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTC30.13489208633093525No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA30.13489208633093525No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA30.13489208633093525No Hit
ACCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT30.13489208633093525No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG30.13489208633093525No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT30.13489208633093525No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT30.13489208633093525No Hit
CACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTTT30.13489208633093525No Hit
GTGATTAGTTAAAAAATAGAACATGCTCGAGAATGACCAGGATGGTTGTG30.13489208633093525No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG30.13489208633093525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)