FastQCFastQC Report
Mon 8 Jan 2024
HNTFHDMXY_n01_MHC-053_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-053_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTATTTTGGACTGGAAGGAATTCAATTTAACTTAACAAATCAGGACA14.0No Hit
AAAAAAGAAAGGGAAAGAAAGGCAATGGCAGGGCTGACTGCCCTGGGCCT14.0No Hit
GCAAGGACGAACTCTGAGCCAAGGCGGGCTCTGGGTTCGTTGGGTTGGGA14.0No Hit
ATCCGACTCAGCGCGAATCTTAAAAAGAAGCGTTAAGTCATAAAACAACA14.0No Hit
GAAAATCTGTGAGAAGAGTGTTCAGAGGCCCTGGACGCCCCCTCCCACAC14.0No Hit
CAATTTAAATGCTGTTCCCTCCTTGTTTCAAGTATAGATCTTAAGGCTAA14.0No Hit
CCAATGTACCATGCCATACTAACTCAACAACAACAAACTAAACATTAATA14.0No Hit
CACTTATCCTATCCTACGGAACAAACCCAATAAATCCCAGCCAGAAAAAC14.0No Hit
CAAGGAAATAGTAAAATAAAAAAAAAGGTTATTTCACTGCACTAGAAGCA14.0No Hit
CCCCAACAAAAAAAAAGTAAAAACACCATAAAATAACAATTCATTCCAAA14.0No Hit
CTCAATTACTACACAATAAGCTCTAACTCAATCAAACCAAAGTTCTAGCA14.0No Hit
ACAAGATAAAACCCACCCCCAGCCATTTAAGTCAACACCTTACATCTAAT14.0No Hit
GTCAATATCCCGAGGGCAGGTGCCCAACATAACAGCATTTTACCTTTTGA14.0No Hit
CCCAGATAAACCCACTAACAAAACATAAGACAGAACGCACAACAAAACCG14.0No Hit
GTGCTGATGAACAAGTTTTTTAATCCTGTAAAATGTTTCCCGCTTTATAG14.0No Hit
CCATGATAAATTCCAATCAACATCAAAAACAAGCAAAAAAGAAGCCTGAA14.0No Hit
GAACATATTACTGAAGATTCCATTTTTGTTTCATGGAGAATTAAAAAAAG14.0No Hit
CCTCATTCAAAAGCCAACTACAGTTGAAAAAGCAGTGCTAAAATGCAGAG14.0No Hit
AATCCAAACATTTCACACAATTTAGTAACAAAGGCTGTATCTCTCTCTCT14.0No Hit
CCCTACCCTTCTCCCTACTTGGAGGCTCTTTATAGGGTGTGACCAGCAGC14.0No Hit
CACCTGTTGACCACAGAAACAGTTCCAAAATCTTCTCCTTGATAGACCAC14.0No Hit
TTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATAC14.0No Hit
CCAGGAAGAGCTTCACTCAAAGCTTCATGGTGCATTTCGACAGATTTTAC14.0No Hit
TGGCCACCTTCATCACTTATTTTAGCTAGGTCTTCTTTCCTTCAACCTCA14.0No Hit
AAAAATTAATAACTTCACCAGGAAGAAAGATACAAAGAAAGAAAATTGAT14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)